Mouse inositol 1,4,5,-trisphosphate(IP3) ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

小鼠1,4,5-三磷酸肌醇(IP3)酶联免疫试剂盒（CSB-E13410m）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆、细胞裂解物样本中的IP3含量。IP3（肌醇三磷酸）是一种重要的细胞信号分子，参与G蛋白偶联受体介导的信号转导。它由PLC水解PIP2产生，能结合内质网上的IP3受体，引发钙离子释放，进而调控细胞内多种生理过程。研究IP3信号通路有助于理解细胞内钙信号调控机制及其在疾病发生中的作用。试剂盒检测范围为0.78 pg/mL-50 pg/mL，适用于实验室研究细胞信号通路激活、受体功能评估或药物干预后IP3动态变化。为探索IP3在生理病理机制中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

IP3
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates, cell lysates
检测范围：

0.78 pg/mL-50 pg/mL
灵敏度：

0.195 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IP3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	86
	Range %	82-95
1:200	Average %	95
	Range %	91-99
1:400	Average %	97
	Range %	96-105
1:800	Average %	98
	Range %	89-102

回收率：

The recovery of mouse IP3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	98-107
EDTA plasma (n=4)	98	92-105

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.