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中文名称：大鼠胰高血糖素样肽1(Glp-1)酶联免疫试剂盒
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货号：CSB-E08117r
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规格：96T/48T
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价格：￥3900/￥2500
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其他：
产品详情
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产品描述：CUSABIO大鼠胰高血糖素样肽1（Glp-1）酶联免疫检测试剂盒（货号CSB-E08117r），基于双抗体夹心法原理实现定量检测，适用于血清、血浆、细胞培养上清液及组织匀浆样本的分析。Glp-1是一种关键的肠促胰岛素激素，通过与受体结合调控胰岛素分泌、抑制胰高血糖素释放并延缓胃排空，在糖代谢稳态和能量平衡研究中具有重要价值。试剂盒检测灵敏度高，线性范围为0.625-40 pg/mL，可精准检测样本中Glp-1的微量表达水平。预包被板及优化的试剂体系确保实验稳定性和重复性，操作流程兼容常规实验室条件。该产品适用于糖尿病机制研究、代谢性疾病模型评估、肠道内分泌功能分析及药物开发中Glp-1相关调控效应的体外验证，为探索胃肠激素信号通路提供可靠工具。需注意本产品仅限于科学研究用途，检测结果不用于临床或诊断目的。
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别名：N/A
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缩写：Glp1
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种属：Rattus norvegicus (Rat)
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样本类型：serum, plasma, cell culture supernates, tissue homogenates
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检测范围：0.625 pg/mL-40 pg/mL
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灵敏度：0.156 pg/mL
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反应时间：1-5h
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样本体积：50-100ul
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检测波长：450 nm
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研究领域：Metabolism
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测定原理：quantitative
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测定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
线性度：
To assess the linearity of the assay, samples were spiked with high concentrations of rat GLP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:100 Average % 86 Range % 84-95 1:200 Average % 95 Range % 91-100 1:400 Average % 84 Range % 81-89 1:800 Average % 92 Range % 86-98 -
回收率：
The recovery of rat GLP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 91 87-96 EDTA plasma (n=4) 103 95-108 -
标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average Corrected 40 2.607 2.744 2.676 2.531 20 2.303 2.451 2.377 2.232 10 1.817 1.922 1.870 1.725 5 1.133 1.191 1.162 1.017 2.5 0.621 0.632 0.627 0.482 1.25 0.344 0.355 0.350 0.205 0.625 0.234 0.248 0.241 0.096 0 0.140 0.150 0.145 -
数据处理：
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货期：3-5 working days
引用文献
- Maternal Diet during Pregnancy Alters the Metabolites in Relation to Metabolic and Neurodegenerative Diseases in Young Adult Offspring SM Kim, S Oh, SS Lee, S Park, YM Hur,International Journal of Molecular Sciences,2024
- Exploring the Role of Duodenal Resurfacing in Diabetes Improvement L Nie,DIABETES OBESITY & METABOLISM,/
- Ginseng extracts improve circadian clock gene expression and reduce inflammation directly and indirectly through gut microbiota and PI3K signaling pathway XY Zhang,NPJ biofilms and microbiomes,2024
- Role of Vildagliptin against Destruction of Pancreatic Beta-Cells in Type 2 Diabetes ACP Salles,/,2024
- Medication adjustment of afatinib and combination therapy with sitagliptin for alleviating afatinib-induced diarrhea in rats L Zhang,Neoplasia,2023
- Layer‐Specific BTX‐A Delivery to the Gastric Muscularis Achieves Effective Weight Control and Metabolic Improvement S Wang,Advanced science,2023
- Yijung-tang improves thermogenesis and reduces inflammation associated with gut microbiota in hypothyroid rats S Khakisahneh,NPJ biofilms and microbiomes,2023
- Sleeve gastrectomy decreased hepatic lipid accumulation by inducing autophagy via AMPK/mTOR pathway R Zeng,Biochemical and biophysical research communications,2023
- Antidiabetic effect of Melicope glabra¬(Blume) TG Hartley (Rutaceae) in high-fat diet/streptozotocin-induced diabetic rats and its bioactive components A Quek,Journal of Food Bioactives,2022
- GLP-1 mediates the neuroprotective action of crocin against cigarette smoking-induced cognitive disorders via suppressing HMGB1-RAGE/TLR4-NF-κB pathway MM El Tabaa,International Immunopharmacology,2022
- Jiangtangjing ameliorates type 2 diabetes through effects on the gut microbiota and cAMP/PKA pathway YM Sun,Traditional Medicine Research,2021
- Repurposing of Omarigliptin as a Neuroprotective Agent Based on Docking with A2A Adenosine and AChE Receptors, Brain GLP-1 Response and Its Brain/Plasma Concentration Ratio after 28 Days Multiple Doses in Rats Using LC-MS/MS Haidy E. Michel,Molecules,2021
- The paradox of dipeptidyl peptidase IV inhibition in enterocytic differentiation and epithelial-mesenchymal transition in rat cholestatic sepsis DA Zaky,Toxicol. Appl. Pharmacol,2020
- Effects of dietary intake of potatoes on body weight gain, satiety-related hormones, and gut microbiota in healthy rats Yu Wu, et al,Rsc Advances,2019
- Sleeve Gastrectomy with Bypass of Proximal Small Intestine Provides Better Diabetes Control than Sleeve Gastrectomy Alone Under Postoperative High-Fat Diet Cheng Y.et al,Obesity Surgery,2018
- Duodenum Exclusion Alone Is Sufficient to Improve Glucose Metabolism in STZ-Induced Diabetes Rats Weihang W.et al,Obesity Surgery,2018
- Influence of Dipeptidyl Peptidase-IV Inhibitor Sitagliptin on Extracellular Signal-Regulated Kinases 1/2 Signaling in Rats with Diabetic Nephropathy Ren X.et al,Pharmacology,2017
- Sitagliptin inhibits endothelin-1 expression in the aortic endothelium of rats with streptozotocin-induced diabetes by suppressing the nuclear factor-κB/IκBα system through the activation of AMP-activated protein kinase Tang S T.et al, International journal of molecular medicine,2016
- Changes in ghrelin, CCK, GLP-1, and peroxisome proliferator-activated receptors in a hypoxia-induced anorexia rat model Duraisamy AJ. et al,Endokrynol Pol,2015
- Inhibiting Roles of Berberine in Gut Movement of Rodents are Related to Activation of the Endogenous Opioid System Feng Y et al,Phytother Res,2013
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