Pig glucagon like peptide 1(GLP1) ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

猪胰高血糖素样肽1(GLP1)酶联免疫试剂盒（CSB-EQ027281PI）为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的GLP1含量。GLP1即胰高血糖素样肽-1，是肠道L细胞分泌的一种肽类激素。它能促进胰岛素分泌、抑制胰高血糖素分泌，延缓胃排空，从而降低血糖。目前基于GLP1的药物在糖尿病、肥胖症等疾病治疗领域研究热度高，为相关治疗带来新方向。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于基础科研领域中对猪模型GLP1分泌调控机制的研究、代谢性疾病动物模型评估、以及相关生物活性分子或药物干预效果的体外检测，为探索GLP1在糖代谢、能量平衡及肠道 - 胰腺轴功能中的作用提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

GLP1
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

3.9 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig GLP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-93
1:2	Average %	95
	Range %	90-105
1:4	Average %	98
	Range %	92-110
1:8	Average %	93
	Range %	85-98

回收率：

The recovery of pig GLP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-104
EDTA plasma (n=4)	94	90-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.