Rat ferritin,FE ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO大鼠铁蛋白（Ferritin, FE）酶联免疫检测试剂盒（货号：CSB-E08826r），采用双抗体夹心法定量检测血清、血浆、组织匀浆及细胞裂解液中的铁蛋白含量。铁蛋白是铁代谢的核心调控蛋白，参与细胞内铁离子的储存与释放，其水平变化与铁稳态失衡、氧化应激及炎症反应等生理病理过程密切相关。试剂盒检测灵敏度为0.9 ng/mL，定量范围覆盖0.9-60 ng/mL，可精准测定低丰度样本。实验操作便捷，适用于科研领域中对铁代谢相关疾病模型的机制研究，如铁过载综合征、缺铁性贫血动物模型的组织或血液样本分析，亦可用于体外细胞实验中铁蛋白表达水平的动态监测，为探索铁代谢调控网络提供可靠工具。试剂盒包含预包被捕获抗体、生物素标记检测抗体及标准品，支持96孔板规格检测，配套试剂优化后显色稳定，重复性良好。
别名：

N/A
缩写：

FE
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates, cell lysates
检测范围：

0.9 ng/mL-60 ng/mL
灵敏度：

0.23 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cancer
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat FE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	84-92
1:2	Average %	94
	Range %	90-98
1:4	Average %	100
	Range %	95-104
1:8	Average %	101
	Range %	96-108

回收率：

The recovery of rat FE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	88-98
EDTA plasma (n=4)	87	82-96

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.