Mouse ferritin,FE ELISA Kit

产品详情

产品描述：

小鼠铁蛋白(FE)酶联免疫试剂盒（CSB-E08827m）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的FE含量。试剂盒检测范围为37.5 ng/mL-2400 ng/mL，适用于探究铁代谢相关分子机制、肝脏功能研究、炎症模型中的铁稳态评估，或细胞实验中铁蛋白表达动态变化的定量监测，为体外实验及动物模型研究提供可靠的数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

N/A
缩写：

FE
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

37.5 ng/mL-2400 ng/mL
灵敏度：

9.4 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cancer
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse FE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	82-99
1:2	Average %	92
	Range %	88-100
1:4	Average %	93
	Range %	85-100
1:8	Average %	91
	Range %	85-105

回收率：

The recovery of mouse FE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-100
EDTA plasma (n=4)	96	85-105

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.