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中文名称：大鼠脑啡肽(ENK)酶联免疫试剂盒
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货号：CSB-E13325r
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规格：96T/48T
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价格：￥3800/￥2500
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其他：
产品详情
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产品描述：大鼠脑啡肽(ENK)酶联免疫试剂盒（CSB-E13325r）为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的ENK含量。试剂盒检测范围为0.625 ng/mL-40 ng/mL，该产品适用于神经科学、药理学等领域的基础研究，例如探究疼痛机制模型、应激反应调控或药物干预对ENK表达的影响，为神经系统功能研究提供可靠的实验工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
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缩写：ENK
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种属：Rattus norvegicus (Rat)
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样本类型：serum, plasma
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检测范围：0.625 ng/mL-40 ng/mL
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灵敏度：0.156 ng/mL
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反应时间：1-5h
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样本体积：50-100ul
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检测波长：450 nm
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研究领域：Neuroscience
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测定原理：quantitative
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测定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
线性度：
To assess the linearity of the assay, samples were spiked with high concentrations of rat ENK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 106 Range % 103-111 1:2 Average % 95 Range % 92-98 1:4 Average % 90 Range % 86-95 1:8 Average % 93 Range % 90-96 -
回收率：
The recovery of rat ENK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 103 99-106 EDTA plasma (n=4) 91 85-95 -
标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
ng/ml OD1 OD2 Average Corrected 40 2.347 2.428 2.388 2.291 20 1.651 1.740 1.696 1.599 10 1.344 1.239 1.292 1.195 5 0.837 0.900 0.869 0.772 2.5 0.485 0.477 0.481 0.384 1.25 0.309 0.289 0.299 0.202 0.625 0.200 0.205 0.203 0.106 0 0.095 0.098 0.097 -
数据处理：
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货期：3-5 working days
引用文献
- Sucrose and naltrexone prevent increased pain sensitivity and impaired long-term memory induced by repetitive neonatal noxious stimulation: Role of BDNF and β-endorphin Nuseir KQ.et al,Physiol Behav.,2017
- Sucrose-induced analgesia during early life modulates adulthood learning and memory formation Khawla Q. Nuseir et al,Physiology & Behavior,2015
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