Human enkephalin,ENK ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CSB-E09117h人脑啡肽（ENK）酶联免疫试剂盒采用竞争法原理，可定量检测血清或血浆样本中的ENK含量，检测灵敏度达0.05 ng/mL，线性范围为0.05-10 ng/mL，适用于科研领域对神经递质调控机制的探索。ENK作为内源性阿片肽，广泛分布于中枢神经系统，参与痛觉调节、情绪反应及免疫调节等生理过程，其水平变化与神经退行性疾病、应激反应及慢性疼痛模型研究密切相关。本试剂盒基于竞争性结合原理，通过样本中的ENK与HRP标记的ENK类似物竞争结合预包被抗体，经显色反应后测定吸光度值，结合标准曲线实现定量分析。实验流程仅需3小时，样本需离心处理并避免反复冻融以保证稳定性，试剂盒提供预包被板、标准品、检测抗体及终止液等全套组分，-20℃保存条件下有效期达12个月。该产品可应用于神经生物学、药理学研究中ENK动态变化的检测，或用于评估药物干预、行为学模型对脑啡肽系统的影响，为探索神经系统功能提供可靠工具。
缩写：

ENK
种属：

Homo sapiens (Human)
样本类型：

serum, plasma
检测范围：

0.05 ng/mL-10 ng/mL
灵敏度：

0.025 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Neuroscience
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human ENK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	92-99
1:2	Average %	93
	Range %	87-100
1:4	Average %	93
	Range %	85-99
1:8	Average %	90
	Range %	82-102

回收率：

The recovery of human ENK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-102
EDTA plasma (n=4)	97	93-105

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.