Rat dynorphin,Dyn ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO专为大鼠样本设计的强啡肽(Dyn)酶联免疫检测试剂盒（货号：CSB-E13294r），采用双抗体夹心法原理实现定量分析，适用于血清、血浆及组织匀浆中Dyn蛋白含量的精准检测。强啡肽作为内源性阿片肽家族成员，广泛分布于中枢神经系统，参与痛觉调控、应激反应及神经内分泌功能调节，是神经生物学与药理学研究的重要靶点。该试剂盒检测灵敏度高，线性范围覆盖2.35至150 pg/mL，可稳定检测低丰度样本，满足不同实验需求。实验操作基于预包被特异性捕获抗体与标记检测抗体的协同作用，通过标准曲线定量目标物浓度，适用于探究疼痛机制、应激生理学、成瘾行为或神经退行性疾病模型中Dyn的动态变化。试剂盒严格验证了血清、血浆（建议EDTA抗凝）及脑、脊髓等组织匀浆的兼容性，为体外研究提供可靠工具，广泛用于神经科学、药物开发及基础医学领域的科研分析。
别名：

N/A
缩写：

Dyn
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

2.35 pg/mL-150 pg/mL
灵敏度：

0.58 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Neuroscience
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat Dyn in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-95
1:2	Average %	102
	Range %	97-107
1:4	Average %	91
	Range %	85-97
1:8	Average %	97
	Range %	91-103

回收率：

The recovery of rat Dyn spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	97	90-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.