Human dynorphin,Dyn ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

人强啡肽（Dynorphin，Dyn）酶联免疫试剂盒（货号：CSB-E09128h）基于双抗体夹心法定量检测人血清、血浆或组织匀浆样本中的Dyn浓度。Dyn是一种内源性阿片肽，主要分布于中枢神经系统，参与痛觉调节、应激反应及情绪调控等生理过程。本试剂盒检测范围为3.12 pg/mL至200 pg/mL，可精准识别低丰度靶标，适用于神经生物学、疼痛机制研究或精神疾病动物模型中的动态监测。实验采用预包被特异性抗体的微孔板，通过生物素标记检测抗体与链霉亲和素-HRP系统显色，结合标准曲线实现定量分析。该试剂盒操作简便，仅需100 μL样本即可完成检测，配套提供标准品、稀释液及终止液等完整组分，适用于实验室规模的高通量筛查或靶向性研究，为探索Dyn在神经信号传导、药物干预效果评估等科研领域提供可靠工具。
别名：

N/A
缩写：

Dyn
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

3.12 pg/mL-200 pg/mL
灵敏度：

0.78 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Neuroscience
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human Dyn in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	80-90
1:2	Average %	95
	Range %	90-100
1:4	Average %	93
	Range %	90-96
1:8	Average %	92
	Range %	87-96

回收率：

The recovery of human Dyn spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	84-99
EDTA plasma (n=4)	100	97-103

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.