Rat bradykinin,BK ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

大鼠血管舒缓激肽(BK)酶联免疫试剂盒（CSB-E06924r）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的BK含量。试剂盒检测范围为0.78 pg/mL-50 pg/mL，本试剂盒适用于基础科研中BK相关信号通路研究、疾病动物模型（如高血压、关节炎、急性肺损伤）的分子机制探索，以及药物干预实验中BK动态变化的定量评估，为心血管系统、炎症反应和神经调节等领域的科研实验提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

BK
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.78 pg/mL-50 pg/mL
灵敏度：

0.195 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat BK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	88
	Range %	84-92
1:10	Average %	94
	Range %	90-98
1:20	Average %	99
	Range %	95-104
1:40	Average %	102
	Range %	96-108

回收率：

The recovery of rat BK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	93-96
EDTA plasma (n=4)	100	95-105

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.