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Human Bradykinin,BK ELISA Kit

文献引用 (2) 说明书下载

中文名称：

人血管舒缓激肽(BK)酶联免疫试剂盒
货号：

CSB-E09155h
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

人血管舒缓激肽(BK)酶联免疫试剂盒（CSB-E09155h）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的BK含量。试剂盒检测范围为0.312 pg/mL-20 pg/mL，适用于心血管疾病机制研究、炎症相关信号通路探索、药物干预效果评估等科研领域；为研究BK在血管内皮功能调控、过敏性休克或局部水肿等病理模型中的作用提供有效工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

BK
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.312 pg/mL-20 pg/mL
灵敏度：

0.078 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human BK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:100		Average %		93
		Range %		90-95
1:200		Average %		103
		Range %		98-107
1:400		Average %		93
		Range %		86-97
1:800		Average %		97
		Range %		91-103

回收率：

The recovery of human BK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type		Average % Recovery		Range
Serum (n=5)		95		91-97
EDTA plasma (n=4)		97		93-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
20	2.586	2.566	2.576	2.467
10	2.224	2.305	2.265	2.156
5	1.711	1.681	1.696	1.587
2.5	1.085	1.077	1.081	0.972
1.25	0.624	0.630	0.627	0.518
0.625	0.379	0.368	0.374	0.265
0.312	0.243	0.232	0.238	0.129
0	0.110	0.108	0.109

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

引用文献

Bradykinin‐bradykinin receptor (B1R) signalling is involved in the blood–brain barrier disruption in moyamoya disease H Wang,Journal of cellular and molecular medicine,2023
Anticoagulant Biomimetic Consecutive Gas Exchange Network for Advanced Artificial Lung Membrane Y Feng,Available at SSRN,2022

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本司产品仅用于科研，不用于临床诊断和治疗

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |