Rat asymmetrical dimethylarginine(ADMA) ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO大鼠不对称二甲基精氨酸（ADMA）酶联免疫检测试剂盒（货号：CSB-E13039r），采用竞争法定量原理，适用于血清、血浆及组织匀浆样本的ADMA含量分析。ADMA作为内源性一氧化氮合酶抑制剂，参与调控血管张力与内皮功能，其水平异常与心血管疾病、代谢综合征及肾脏疾病等病理机制密切相关，是科研领域研究氧化应激与炎症反应的重要生物标志物。试剂盒检测范围为1.5-300 ng/mL，通过样本中的ADMA与酶标记物竞争结合特异性抗体的原理实现定量，显色信号强度与ADMA浓度呈反比，可精准检测微量样本中的目标物质。本试剂盒适用于心血管疾病模型研究、代谢紊乱机制探索、肾脏功能评估等基础科研实验，也可用于药物干预或基因调控实验中ADMA水平变化的动态监测，为动物模型病理机制解析及治疗策略开发提供可靠数据支持。实验操作简便，兼容常规实验室设备，满足科研场景下对ADMA的高灵敏度检测需求。
缩写：

ADMA
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

1.5 ng/mL-300 ng/mL
灵敏度：

0.75 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat ADMA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	85-93
1:2	Average %	92
	Range %	86-96
1:4	Average %	95
	Range %	90-101
1:8	Average %	103
	Range %	99-106

回收率：

The recovery of rat ADMA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	94-104
EDTA plasma (n=4)	84	80-89

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.