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中文名称:大鼠晚期糖基化终末产物(AGEs)酶联免疫试剂盒
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货号:CSB-E09413r
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规格:96T/48T
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价格:¥3900/¥2500
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其他:
产品详情
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产品描述:CUSABIO大鼠晚期糖基化终末产物(AGEs)检测提供高灵敏度解决方案,AGEs是由蛋白质或脂质等生物大分子通过非酶糖基化反应生成的稳定化合物,其异常积累与糖尿病并发症、衰老进程及慢性炎症性疾病的发生发展密切相关。该试剂盒采用双抗体夹心法原理进行定量分析,可精准检测血清、血浆及组织匀浆样本中的AGEs浓度,线性检测范围覆盖3.9-250 μg/mL,最低检测限达3.9 μg/mL,能够满足不同浓度样本的检测需求。产品配套标准品和优化反应体系,操作流程标准化且重复性良好,适用于糖尿病动物模型研究、衰老相关机制探索、氧化应激水平评估等科研领域,尤其适合在心血管疾病、神经退行性疾病等慢性病理模型的建立与验证中,通过血清、血浆或组织样本的动态监测揭示AGEs的生物学作用。实验全程无需特殊设备,常规酶标仪即可完成读数,为科研工作者提供高效便捷的AGEs定量检测方案。
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缩写:AGEs
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种属:Rattus norvegicus (Rat)
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样本类型:serum, plasma, tissue homogenates
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检测范围:3.9 μg/mL-250 μg/mL
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灵敏度:0.97 μg/mL
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反应时间:1-5h
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样本体积:50-100ul
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检测波长:450 nm
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研究领域:Immunology
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测定原理:quantitative
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测定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rat AGEs in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:5 Average % 86 Range % 84-95 1:10 Average % 95 Range % 91-100 1:20 Average % 85 Range % 81-89 1:40 Average % 93 Range % 89-96 -
回收率:
The recovery of rat AGEs spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 91 87-96 EDTA plasma (n=4) 95 90-100 -
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. μg/ml OD1 OD2 Average Corrected 250 2.194 2.286 2.240 2.084 125 1.764 1.789 1.777 1.621 62.5 1.270 1.256 1.263 1.107 31.2 0.921 0.975 0.948 0.792 15.6 0.455 0.465 0.460 0.304 7.8 0.297 0.308 0.303 0.147 3.9 0.248 0.233 0.241 0.085 0 0.161 0.150 0.156 -
数据处理:
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货期:3-5 working days
引用文献
- Antioxidant and Hepatoprotective Effects of Moringa oleifera-mediated Selenium Nanoparticles in Diabetic Rats. AA Ahmad Tarmizi, NN Nik Ramli,F1000Research,2025
- Okanin alleviates symptoms of nociceptive-like responses in diabetic peripheral neuropathy in type 1 diabetic Wistar rats by regulating the AGEs/NF-κB/Nrf-2 pathway MR Ganie, N Khan, M Shukla, S Sood, S Devi,Journal of Pharmacological Sciences,2024
- Carthamus tinctorius L. (Safflower) Flower Extract Attenuates Hepatic Injury and Steatosis in a Rat Model of Type 2 Diabetes Mellitus via Nrf2-Dependent Hypoglycemic, Antioxidant, and Hypolipidemic Effects NS Alshareef, SA AlSedairy, LN Al-Harbi,Antioxidants,2024
- Comparative Efficacy of Low-Carbohydrate and Ketogenic Diets on Diabetic Retinopathy and Oxidative Stress in High-Fat Diet-Induced Diabetic Rats MT Jawharji, GM Alshammari, MA Binobead,Nutrients,2024
- Phloretamide Protects against Diabetic Kidney Damage and Dysfunction in Diabetic Rats by Attenuating Hyperglycemia and Hyperlipidemia, Suppressing NF-κβ, and Upregulating Nrf2 R Al,Pharmaceutics,2024
- Renoprotective impact of Dapagliflozin and Mulberry extracts toward Fr-STZ induced diabetic nephropathy in rats: Biochemical and Molecular aspects EM Gad,researchsquare,2023
- Neuroprotective Activity of Eriodictyol Against Streptozotocin-Induced Diabetic Peripheral Neuropathy in Wistar Rats by Targeting Wnt/β-Catenin Pathway R Malik,researchsquare,2023
- A standardized pomegranate fruit extract ameliorates thioacetamide-induced liver fibrosis in rats via AGE-RAGE-ROS signaling HM Abouelezz,Heliyon,2023
- Role of Klotho and AGE/RAGE-Wnt/β-Catenin Signalling Pathway on the Development of Cardiac and Renal Fibrosis in Diabetes B Martín-Carro,International Journal of Molecular Sciences,2023
- DL0410 Alleviates Memory Impairment in D-Galactose-Induced Aging Rats by Suppressing Neuroinflammation via the TLR4/MyD88/NF-κB Pathway B Zhang,Oxid Med Cell Longev,2021
- Activation of RAGE-dependent endoplasmic reticulum stress associates with exacerbated post-myocardial infarction ventricular arrhythmias in diabetes Z Liu,American Journal of Physiology-Endocrinology and Metabolism,2021
- Astragaloside IV Protects Against Diabetic Nephropathy by Inhibiting Pyroptosis Based on NOX4/TXNIP/NLRP3 Pathway Y Zhang,Authorea,2020
- Transcriptomic Analysis Reveals the Protection of Astragaloside IV against Diabetic Nephropathy by Modulating Inflammation Y Zhang,Oxid Med Cell Longev,2020
- Standardised Extracts of Moringa Oleifera and Centella Asiatica Enhanced the Antioxidant Activity, Learning and Memory Effects by Inhibiting … H Jamari,Pertanika J. Sci. & Technol.,2020
- Effects and mechanisms of PSS-loaded nanoparticles on coronary microcirculation dysfunction in streptozotocin-induced diabetic cardiomyopathy rats Mao Y, et al,Biomedicine & Pharmacotherapy,2019
- Integrated proteomics, biological functional assessments and metabolomics reveal toosendanin induced hepatic energy metabolic disorders Yan X, et al,Chemical research in toxicology,2019
- Analysis of differentially expressed advanced glycation end product-modified proteins in diabetic rat kidney Jingfang Liu.et al,International Journal of Diabetes in Developing Countries,2018
- GS-E3D, a new pectin lyase-modified red ginseng extract, inhibited diabetes-related renal dysfunction in streptozotocin-induced diabetic rats Kim CS.et al,BMC Complement Altern Med.,2017
- Antiglycation and Hypolipidemic Effects of Polyphenols from Zingiber officinale Roscoe (Zingiberaceae) in Streptozotocin-Induced Diabetic Rats Mutiu I Kazeem et al,Tropical Journal of Pharmaceutical Research January,2015
- Advanced glycation end products (AGE) induce the receptor for AGE in the colonic mucosa of azoxymethane-injected Fischer 344 rats fed with a high-linoleic acid and high-glucose diet /,Journal of Gastroenterology,2012
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相关问答
我想从贵公司订购“rat advanced glycation end products (AGEs) ELISA Kit (#CSB-E09413r)”,但我想了解以下信息: Q1,是否有数据说明为什么我们可以根据宿主物种选择AGEs Elisa试剂盒?由于AGEs没有特异性表位,我认为宿主物种并不是确定AGEs抗体特异性的关键因素。 Q2.如果我使用大鼠AGEs ELISA kit来分析小鼠组织样本,很难保证该试剂盒的特异性或数据质量问题吗?
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