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Human advanced glycation end products,AGEs ELISA Kit

  • 中文名称:
    人晚期糖基化终末产物(AGEs)酶联免疫试剂盒
  • 货号:
    CSB-E09412h
  • 规格:
    96T/48T
  • 价格:
    ¥3600/¥2500
  • 其他:

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  • 产品描述:
    CUSABIO人晚期糖基化终末产物(AGEs)酶联免疫检测试剂盒(货号:CSB-E09412h),采用双抗体夹心法定量检测血清、血浆或组织匀浆样本中的AGEs含量,检测灵敏度为0.78 μg/mL,线性范围为0.78-50 μg/mL。AGEs是蛋白质或脂质经非酶促糖基化反应形成的稳定化合物,在糖尿病并发症、衰老相关疾病及慢性炎症中具有重要病理意义,其累积与氧化应激、血管功能障碍及组织损伤密切相关。本试剂盒基于特异性抗体对捕获和检测靶标,适用于科研场景中AGEs相关机制研究,例如糖尿病模型动物血清/组织样本分析、体外药物干预对AGEs生成抑制效果的评估,或阿尔茨海默病等神经退行性疾病中AGEs沉积的病理学关联探索。实验操作全程约4小时完成,配套提供标准品、预包被酶标板及检测所需试剂,支持科研人员高效开展AGEs在代谢性疾病、慢性炎症或衰老机制中的基础研究与体外实验数据获取。
  • 缩写:
    AGEs
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    0.78 μg/mL-50 μg/mL
  • 灵敏度:
    0.195 μg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Immunology
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human AGEs in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %84
    Range %80-89
    1:2Average %90
    Range %85-95
    1:4Average %94
    Range %90-99
    1:8Average %104
    Range %100-109
  • 回收率:
    The recovery of human AGEs spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9288-96
    EDTA plasma (n=4)9792-101
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    µg/mlOD1OD2AverageCorrected
    502.698 2.652 2.675 2.534
    252.076 2.082 2.079 1.938
    12.51.204 1.283 1.244 1.103
    6.250.639 0.669 0.654 0.513
    3.120.388 0.374 0.381 0.240
    1.560.261 0.259 0.260 0.119
    0.780.187 0.179 0.183 0.042
    00.145 0.137 0.141  
  • 本试剂盒所含材料:
    • A micro ELISA plate --- The 96-well plate has been pre-coated with anti-human AGEs antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
    • Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • One vial Biotin-labeled AGEs antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
    • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody solution.
    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
    • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
    • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
    • One vial TMB Substrate (10 ml/bottle) --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the chromogenic reaction.
    • One vial Stop Solution (10 ml/bottle) --- Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
    • An instruction manual

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  • 本试剂盒不含材料:
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 数据处理:
  • 货期:
    3-5 working days

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相关问答

 常见问题解答
Q:

I would like to know if this kit can evaluate all AGEs or only a specific one, like arboximetilisina.

A:
Thanks for your inquiry.
This kit quantifys all AGEs.