Rat Thromboxane B2,TXB2 ELISA Kit

Rat Thromboxane B2,TXB2 ELISA Kit

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中文名称：

大鼠血栓素B2(TXB2)酶联免疫试剂盒
货号：

CSB-E08047r
规格：

96T/48T
价格：

￥3900/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO大鼠血栓素B2（TXB2）酶联免疫检测试剂盒（货号：CSB-E08047r），采用双抗体夹心法定量检测血清、血浆或组织匀浆样本中的TXB2含量，检测灵敏度达3.9 pg/mL，线性范围为3.9-250 pg/mL。TXB2是血栓素A2（TXA2）的稳定水解产物，作为重要的脂质介质参与血小板活化、血管收缩及炎症反应调控，其水平变化与血栓性疾病、动脉粥样硬化及组织损伤修复等病理过程密切相关。本试剂盒通过特异性抗体对靶标分子进行高精度捕获与检测，适用于心血管疾病动物模型研究、抗血小板药物药效评估、炎症相关机制探索等科研领域。操作流程包括样本预处理、标准品/样本孵育、酶标抗体结合及显色反应，配套提供标准曲线建立所需的预包被板条与优化试剂，实验人员可根据样本类型灵活选择检测方案，满足基础研究中对TXB2动态变化的定量分析需求。
缩写：

TXB2
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

3.9 pg/mL-250 pg/mL
灵敏度：

0.97 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat TXB2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	88-98
1:2	Average %	95
	Range %	91-102
1:4	Average %	99
	Range %	94-106
1:8	Average %	93
	Range %	87-97

回收率：

The recovery of rat TXB2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	90-103
EDTA plasma (n=4)	92	88-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
250	1.990	1.890	1.940	1.785
125	1.298	1.198	1.248	1.093
62.5	0.786	0.766	0.776	0.621
31.25	0.545	0.525	0.535	0.380
15.6	0.341	0.321	0.331	0.176
7.8	0.292	0.272	0.282	0.127
3.9	0.215	0.211	0.213	0.058
0	0.156	0.154	0.155

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.