Mouse Thromboxane B2,TXB2 ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

小鼠血栓素B2(TXB2)酶联免疫试剂盒（CSB-E08048m）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TXB2含量。TXB2 即血栓素 B2，是血栓素 A2 的稳定水解产物。它在体内参与血小板聚集和血管收缩过程。研究机制方面，常通过检测其水平反映体内血栓素 A2 活性，以评估血小板功能、血管内皮损伤及血栓形成倾向，在心血管疾病等研究中受关注。试剂盒检测范围为2.35 pg/mL-150 pg/mL，其水平变化常被用于评估血栓形成、心血管疾病及炎症相关机制的研究；适用于心血管疾病模型、炎症机制研究、抗血小板药物筛选等科研领域，可为探索病理生理过程中TXB2的动态变化及其分子调控网络提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

TXB2
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

2.35 pg/mL-150 pg/mL
灵敏度：

0.58 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse TXB2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	99
	Range %	95-104
1:2	Average %	89
	Range %	84-94
1:4	Average %	90
	Range %	85-96
1:8	Average %	98
	Range %	93-103

回收率：

The recovery of mouse TXB2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	94-102
EDTA plasma (n=4)	91	86-97

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.