Rat Serum amyloid A,SAA ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO定量检测大鼠血清淀粉样蛋白A（SAA）的酶联免疫试剂盒，采用双抗体夹心法原理，能够灵敏、特异地分析血清、血浆及组织匀浆样本中的SAA含量。SAA是一种重要的急性期反应蛋白，主要由肝脏合成并在感染、创伤或炎症过程中显著升高，可作为评估机体炎症状态及疾病进展的关键生物标志物。试剂盒检测范围为78 ng/mL至5000 ng/mL，标准曲线线性稳定，满足低丰度与高浓度样本的精准检测需求。实验操作流程简便，包含预包被捕获抗体的96孔板、生物素标记检测抗体及HRP标记链霉亲和素系统，通过显色反应实现目标蛋白的定量分析。该试剂盒适用于炎症机制研究、感染性疾病模型评估、免疫调节药物开发等科研领域，例如在实验性关节炎、脓毒症或肿瘤模型中动态监测SAA水平变化，为病理机制解析及治疗干预效果评价提供可靠数据支持。试剂盒所有组分均经过严格质控，开封后稳定性良好，配套详细操作手册及标准品，确保实验可重复性与数据准确性。
缩写：

SAA
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

78 ng/mL-5000 ng/mL
灵敏度：

19.5 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat SAA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	93-99
1:2	Average %	94
	Range %	91-97
1:4	Average %	100
	Range %	97-103
1:8	Average %	102
	Range %	99-105

回收率：

The recovery of rat SAA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	82-91
EDTA plasma (n=4)	98	95-101

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.