Pig serum amyloid A,SAA ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO猪血清淀粉样蛋白A（SAA）酶联免疫检测试剂盒（货号：CSB-E13309p），采用竞争法定量原理，适用于检测猪血清或血浆样本中的SAA蛋白浓度。SAA是一种由肝脏合成的急性期反应蛋白，在炎症、感染或组织损伤时显著升高，可作为评估机体免疫状态的重要生物标志物。该试剂盒检测灵敏度高，线性范围为19.53-5000 pg/mL，通过预包被特异性抗体的微孔板与样本中的SAA竞争结合标记物实现定量分析。试剂盒包含标准品、检测抗体、显色底物等全套组分，操作流程标准化，适用于科研领域中对猪免疫应答机制、感染性疾病模型或疫苗效果评估等研究。通过精确检测SAA水平，研究者可动态监测实验动物炎症反应进程，为畜牧养殖健康管理、病理机制探索及药物干预效果分析提供数据支持。本产品需配合酶标仪（450nm波长）使用，实验全程需按说明书规范操作以确保结果可靠性。
别名：

SAA2; Serum amyloid A-2 protein
缩写：

SAA
Uniprot No.：

P0DSO0
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma
检测范围：

19.53 pg/mL-5000 pg/mL
灵敏度：

19.53 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig SAA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	97
	Range %	92-103
1:2000	Average %	95
	Range %	91-99
1:4000	Average %	101
	Range %	98-105
1:8000	Average %	95
	Range %	86-96

回收率：

The recovery of pig SAA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	88-99
EDTA plasma (n=4)	103	98-107

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.