Rat Platelet-derived growth factor CC(PDGF-CC) ELISA kit

产品详情

产品描述：

大鼠血小板衍生生长因子CC(PDGF-CC) 酶联免疫试剂盒（CSB-EL017710RA）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PDGF-CC含量。PDGF - CC即血小板衍生生长因子CC，是PDGF家族成员。它在胚胎发育、组织修复等过程有重要作用。其异常表达与肿瘤、纤维化等疾病相关。研究机制多围绕其与受体结合，激活下游信号通路，影响细胞增殖、迁移等方面。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于科研领域对大鼠模型中PDGF-CC蛋白表达的定量分析，例如在心血管疾病、组织再生或肿瘤机制研究中评估目标分子动态变化本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

PDGF-CC
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

3.9 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat PDGF-CC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	85-95
1:2	Average %	103
	Range %	99-112
1:4	Average %	98
	Range %	94-102
1:8	Average %	90
	Range %	85-94

回收率：

The recovery of rat PDGF-CC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	82-93
EDTA plasma (n=4)	95	90-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.