Human platelet-derived growth factor CC (PDGF-CC) ELISA Kit
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中文名称:人血小板衍生生长因子CC(PDGF-CC)酶联免疫试剂盒
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货号:CSB-E13241h
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规格:96T/48T
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价格:¥3600/¥2500
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其他:
产品详情
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产品描述:人血小板衍生生长因子CC(PDGF-CC)酶联免疫试剂盒(CSB-E13241h)为双抗夹心法ELISA试剂盒,定量检测血清、组织培养上清液、尿液、组织匀浆样本中的PDGF-CC含量。PDGF-CC 即血小板衍生生长因子 CC,在细胞生长、增殖等生理过程中发挥作用。其异常表达与肿瘤、纤维化等疾病的发生发展相关。研究机制主要聚焦于其信号通路,阻断该信号有望为相关疾病治疗提供方向,目前是研究热门靶点。试剂盒检测范围为0.156 ng/mL-10 ng/mL,科研人员可通过该产品开展PDGF-CC在疾病模型中的表达水平监测、药物干预效果评估或信号通路机制研究,为心血管生物学、肿瘤微环境及再生医学等领域的体外实验提供可靠数据支持。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
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缩写:PDGF-CC
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种属:Homo sapiens (Human)
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样本类型:serum, cell culture supernates, urine, tissue homogenates
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检测范围:0.156 ng/mL-10 ng/mL
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灵敏度:0.039 ng/mL
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反应时间:1-5h
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样本体积:50-100ul
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检测波长:450 nm
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研究领域:Cardiovascular
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测定原理:quantitative
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测定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human PDGF-CC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 92 Range % 88-96 1:2 Average % 95 Range % 92-99 1:4 Average % 94 Range % 89-101 1:8 Average % 89 Range % 84-95 -
回收率:
The recovery of human PDGF-CC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 96 91-102 -
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 10 2.308 2.418 2.363 2.260 5 1.887 2.032 1.960 1.857 2.5 1.452 1.423 1.438 1.335 1.25 0.782 0.821 0.802 0.699 0.625 0.477 0.497 0.487 0.384 0.312 0.325 0.346 0.336 0.233 0.156 0.215 0.205 0.210 0.107 0 0.104 0.101 0.103 -
数据处理:
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货期:3-5 working days
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