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Human platelet-derived growth factor CC (PDGF-CC) ELISA Kit

  • 中文名称:
    人血小板衍生生长因子CC(PDGF-CC)酶联免疫试剂盒
  • 货号:
    CSB-E13241h
  • 规格:
    96T/48T
  • 价格:
    ¥3600/¥2500
  • 其他:

产品详情

  • 产品描述:
    人血小板衍生生长因子CC(PDGF-CC)酶联免疫试剂盒(CSB-E13241h)为双抗夹心法ELISA试剂盒,定量检测血清、组织培养上清液、尿液、组织匀浆样本中的PDGF-CC含量。PDGF-CC 即血小板衍生生长因子 CC,在细胞生长、增殖等生理过程中发挥作用。其异常表达与肿瘤、纤维化等疾病的发生发展相关。研究机制主要聚焦于其信号通路,阻断该信号有望为相关疾病治疗提供方向,目前是研究热门靶点。试剂盒检测范围为0.156 ng/mL-10 ng/mL,科研人员可通过该产品开展PDGF-CC在疾病模型中的表达水平监测、药物干预效果评估或信号通路机制研究,为心血管生物学、肿瘤微环境及再生医学等领域的体外实验提供可靠数据支持。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 缩写:
    PDGF-CC
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, cell culture supernates, urine, tissue homogenates
  • 检测范围:
    0.156 ng/mL-10 ng/mL
  • 灵敏度:
    0.039 ng/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Cardiovascular
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human PDGF-CC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %92
    Range %88-96
    1:2Average %95
    Range %92-99
    1:4Average %94
    Range %89-101
    1:8Average %89
    Range %84-95
  • 回收率:
    The recovery of human PDGF-CC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9691-102
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    102.308 2.418 2.363 2.260
    51.887 2.032 1.960 1.857
    2.51.452 1.423 1.438 1.335
    1.250.782 0.821 0.802 0.699
    0.6250.477 0.497 0.487 0.384
    0.3120.325 0.346 0.336 0.233
    0.1560.215 0.205 0.210 0.107
    00.104 0.101 0.103  
     
  • 数据处理:
  • 货期:
    3-5 working days

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