Rat Nesfatin-1 ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO大鼠神经肽Nesfatin-1（Nesfatin-1）酶联免疫检测试剂盒（货号：CSB-E14378r），采用双抗体夹心ELISA原理实现定量检测，可精准分析血清、血浆及组织匀浆样本中Nesfatin-1的含量，检测灵敏度达15.6 pg/mL，线性范围为15.6-1000 pg/mL。Nesfatin-1是一种广泛分布于中枢神经系统和外周组织的神经肽，参与调控摄食行为、能量代谢及应激反应，其水平变化与肥胖、代谢综合征等研究密切相关。本试剂盒包含预包被捕获抗体的96孔板、生物素标记检测抗体、系列浓度标准品、HRP标记链霉亲和素及显色底物，通过标准曲线法实现目标蛋白的准确定量，适用于神经生物学、代谢调控机制研究，以及肥胖相关动物模型的病理机制探索。所有组分均经过严格验证，确保批次间稳定性，试剂盒组分需按说明书要求保存于4°C或-20°C，避免反复冻融。本产品操作流程标准化，适用于实验室规模的样本批量检测，为研究Nesfatin-1在生理及病理过程中的作用提供可靠工具。
缩写：

Nesfatin-1
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

3.9 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat nesfatin-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-92
1:2	Average %	89
	Range %	85-95
1:4	Average %	93
	Range %	89-99
1:8	Average %	95
	Range %	90-100

回收率：

The recovery of rat nesfatin-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	97-109
EDTA plasma (n=4)	97	93-102

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.