Human Nesfatin-1 ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

本试剂盒（CSB-E15050h）采用双抗体夹心法原理，专为定量检测人血清、血浆及组织匀浆中的神经肽Nesfatin-1设计。Nesfatin-1是一种由核组蛋白2编码的多肽，广泛分布于下丘脑、胃肠道等组织，参与调控食欲、能量代谢及应激反应等生理过程。该试剂盒检测灵敏度为15.6 pg/mL，在31.25-2000 pg/mL线性范围内可实现精准定量分析，实验全程仅需3.5小时，配备预包被抗体、冻干标准品（2瓶）及96孔微孔板。样本兼容性强，适用于代谢疾病模型研究、肥胖相关神经内分泌机制探索，或通过检测脑组织、外周血等样本动态观察Nesfatin-1在应激状态下的表达变化。其高特异性可有效避免交叉反应干扰，为研究能量代谢调控、神经肽信号通路提供可靠工具，配套实验方案经优化适配复杂生物样本，满足基础科研中对低丰度靶标的高效检测需求。
缩写：

Nesfatin-1
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

31.25 pg/mL-2000 pg/mL
灵敏度：

7.81 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human Nesfatin-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	96
	Range %	80-105
1:10	Average %	99
	Range %	91-111
1:20	Average %	104
	Range %	92-112
1:40	Average %	93
	Range %	86-100

回收率：

The recovery of human Nesfatin-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-98
EDTA plasma (n=4)	97	90-107

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.