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Human Nesfatin-1 ELISA Kit

  • 中文名称:
    人神经肽Nesfatin-1酶联免疫试剂盒
  • 货号:
    CSB-E15050h
  • 规格:
    96T/48T
  • 价格:
    ¥3200/¥2500
  • 其他:

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  • 产品描述:
    本试剂盒(CSB-E15050h)采用双抗体夹心法原理,专为定量检测人血清、血浆及组织匀浆中的神经肽Nesfatin-1设计。Nesfatin-1是一种由核组蛋白2编码的多肽,广泛分布于下丘脑、胃肠道等组织,参与调控食欲、能量代谢及应激反应等生理过程。该试剂盒检测灵敏度为15.6 pg/mL,在31.25-2000 pg/mL线性范围内可实现精准定量分析,实验全程仅需3.5小时,配备预包被抗体、冻干标准品(2瓶)及96孔微孔板。样本兼容性强,适用于代谢疾病模型研究、肥胖相关神经内分泌机制探索,或通过检测脑组织、外周血等样本动态观察Nesfatin-1在应激状态下的表达变化。其高特异性可有效避免交叉反应干扰,为研究能量代谢调控、神经肽信号通路提供可靠工具,配套实验方案经优化适配复杂生物样本,满足基础科研中对低丰度靶标的高效检测需求。
  • 缩写:
    Nesfatin-1
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    31.25 pg/mL-2000 pg/mL
  • 灵敏度:
    7.81 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Others
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human Nesfatin-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:5Average %96
    Range %80-105
    1:10Average %99
    Range %91-111
    1:20Average %104
    Range %92-112
    1:40Average %93
    Range %86-100
  • 回收率:
    The recovery of human Nesfatin-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9489-98
    EDTA plasma (n=4)9790-107
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    20002.617 2.557 2.587 2.463
    10002.205 2.356 2.281 2.157
    5001.636 1.767 1.702 1.578
    2501.043 1.058 1.051 0.927
    1250.609 0.642 0.626 0.502
    62.50.352 0.346 0.349 0.225
    31.250.226 0.243 0.235 0.111
    00.122 0.125 0.124  
  • 数据处理:
  • 货期:
    3-5 working days

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