Rat Mucin-5 subtype AC(MUC5AC)ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO大鼠粘蛋白/粘液素5AC（MUC5AC）酶联免疫检测试剂盒（货号：CSB-E16223r），采用双抗体夹心法定量检测样本中MUC5AC含量。MUC5AC是黏蛋白家族重要成员，主要分布于呼吸道和消化道黏膜上皮细胞分泌的黏液中，参与黏液屏障形成及病原体防御，其异常表达与慢性炎症、纤维化等病理过程密切相关。本试剂盒可适用于血清、血浆、细胞培养上清及组织匀浆样本的检测，检测灵敏度为62.5 pg/mL，线性范围为62.5-4000 pg/mL，通过高特异性抗体实现对目标蛋白的精准捕获与定量分析。该试剂盒适用于基础科研领域，例如研究呼吸道疾病模型（如哮喘、慢性阻塞性肺病）中黏液分泌调控机制，评估胃肠道黏膜屏障功能变化，或探索药物、环境因素对黏液代谢通路的影响，为相关生物标志物研究及分子机制解析提供可靠工具。试剂盒操作便捷，配套标准品及优化缓冲体系确保实验稳定性和重复性，适用于实验室常规检测及高通量筛查需求。
缩写：

MUC5AC
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

62.5 pg/mL-4000 pg/mL
灵敏度：

15.6 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat MUC5AC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	94
	Range %	87-97
1:10	Average %	97
	Range %	92-102
1:20	Average %	92
	Range %	88-97
1:40	Average %	95
	Range %	89-100

回收率：

The recovery of rat MUC5AC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	85-95
EDTA plasma (n=4)	99	95-106

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.