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中文名称:大鼠褪黑素(MT)酶联免疫试剂盒
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货号:CSB-E13433r
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规格:96T/48T
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价格:¥3800/¥2500
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其他:
产品详情
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产品描述:CUSABIO大鼠褪黑素(Melatonin, MT)酶联免疫检测试剂盒(货号:CSB-E13433r),采用竞争法原理实现定量分析,适用于血清、血浆样本的科研检测。褪黑素作为主要由松果体分泌的吲哚类激素,在调节昼夜节律、抗氧化应激及免疫调节中发挥重要作用,其浓度变化常作为神经内分泌研究和动物模型评估的重要指标。试剂盒检测灵敏度高,线性范围覆盖12.5至500 pg/mL,可精准检测生物样本中MT的动态水平。实验操作采用预包被抗体和生物素标记抗原的竞争反应体系,通过酶标二抗催化显色反应实现定量测定,具备良好的重复性和特异性。该产品适用于昼夜节律机制研究、睡眠障碍动物模型评估、神经退行性疾病相关实验,以及环境压力或药物干预下激素水平变化的科研分析,为探索MT在生理调控、衰老机制和免疫代谢中的作用提供可靠工具。需注意试剂盒组分包含标准品、检测板、洗涤缓冲液等核心实验材料,建议按照操作手册进行样本前处理及实验流程控制。
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别名:N/A
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缩写:MT
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种属:Rattus norvegicus (Rat)
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样本类型:serum, plasma
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检测范围:12.5 pg/mL-500 pg/mL
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灵敏度:12.5 pg/mL
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反应时间:1-5h
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样本体积:50-100ul
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检测波长:450 nm
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研究领域:Epigenetics and Nuclear Signaling
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测定原理:quantitative
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测定方法:Competitive
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精密度:
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
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标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml
OD1
OD2
Average
500
0.321
0.334
0.328
250
0.687
0.667
0.677
125
0.976
0.928
0.952
42
1.487
1.529
1.508
12.5
1.836
1.821
1.829
0
2.155
2.253
2.204
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数据处理:
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货期:3-5 working days
引用文献
- Therapeutic effects of melatonin on the lungs of rats exposed to passive smoking J Xiong, L Xie, YR Huang, JH Zhu, ZY Hong,Respiratory Research,2024
- Pineal stones have an important function in Alzheimer's disease W Ge,/,2024
- Melatonin improves cardiac remodeling and brain‐heart sympathetic hyperactivation aggravated by light disruption after myocardial infarction L Jiao,Journal of pineal research,2022
- The Circadian Clock is Essential for the Crosstalk of VEGF-Notch-mediated Endothelial Angiogenesis in Ischemic Stroke Y Zhang,ResearchSquare,2022
- A pharmacological composition for induction of a reversible torpor-like state and hypothermia in rats Zakharova NM, et al,Life Sciences,2019
- Thymulin, free or bound to PBCA nanoparticles, protects mice against chronic septic inflammation Elena G.et al,PLOS ONE,2018
- Micronuclei Formation and 8-Hydroxy-2-Deoxyguanosine Enzyme Detection in Ovarian Tissues After Radiofrequency Exposure at 1800 MHz in Adult Sprague?Dawley Rats Ali Saeed Al-Chalabi.et al,HAYATI Journal of Biosciences,2017
- Cardioprotective Effects of HuoxueAnshen Recipe against Myocardial Injuries Induced by Sleep Deprivation in Rats Rong Yuan.et al,Evidence-Based Complementary and Alternative Medicine,2017
- Evaluation of effects of melatonin and caffeic acid phenethyl ester on acute potassium dichromate toxicity and genotoxicity in rats Mujgan Cengiz.et al,Indian journal of pharmacology,2016
- Different periods of intrauterine exposure to electromagnetic field: Influence on female rats' fertility, prenatal and postnatal development Alchalabi A S H.et al,Asian Pacific Journal of Reproduction,2016
- Effect of circadian rhythm disturbance on morphine preference and addiction in male rats: Involvement of period genes and dopamine D1 receptor Garmabi B. et al,Neuroscience.,2016
- Different periods of intrauterine exposure to electromagnetic field: Influence on female rats' fertility, prenatal and postnatal development Ali S.H. Alchalabi.et al,Asian Pacific Journal of Reproduction,2016
- Protective effect of melatonin against inequality-induced damages on testicular tissue and sperm parameters Nasiraei-Moghadam SN et al,Int J Fertil Steril,2014
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