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Human Melatonin,MT ELISA Kit

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CUSABIO人褪黑素（Melatonin, MT）酶联免疫检测试剂盒（货号CSB-E08132h），采用双抗体夹心法定量检测血清、血浆及组织匀浆样本中的MT浓度。褪黑素是由松果体分泌的重要激素，参与调控昼夜节律、睡眠觉醒周期，并具有抗氧化及免疫调节功能。试剂盒检测范围为6.25-400 pg/mL，最低检测限为6.25 pg/mL，适用于微量样本的精准分析。实验基于预包被特异性抗体的固相载体，通过生物素标记检测抗体与目标蛋白结合，结合链霉亲和素-HRP系统催化显色反应，最终通过标准曲线实现定量检测。该试剂盒兼容多种生物样本，可应用于神经生物学、睡眠研究、内分泌调控机制等科研领域，例如探究昼夜节律紊乱模型中的激素水平变化，或评估药物干预对褪黑素分泌的影响。试剂盒包含预包被96孔板、标准品、检测抗体及配套试剂，操作流程优化后可在4.5小时内完成检测，为科研工作者提供稳定、高效的实验工具。
别名：

N/A
缩写：

MT
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

6.25 pg/mL-400 pg/mL
灵敏度：

1.56 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Epigenetics and Nuclear Signaling
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human MT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-88
1:2	Average %	99
	Range %	92-106
1:4	Average %	88
	Range %	82-93
1:8	Average %	90
	Range %	84-94

回收率：

The recovery of human MT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-99
EDTA plasma (n=4)	103	95-110

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
400	2.451	2.357	2.404	2.323
200	1.627	1.616	1.622	1.541
100	1.046	1.003	1.025	0.944
50	0.437	0.473	0.455	0.374
25	0.274	0.285	0.280	0.199
12.5	0.189	0.196	0.193	0.112
6.25	0.143	0.141	0.142	0.061
0	0.082	0.079	0.081

本试剂盒所含材料：
- A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human MT antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled MT antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
- One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) --- Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
- An instruction manual
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本试剂盒不含材料：
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.