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Rat Lipopolysaccharides(LPS) ELISA Kit

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产品描述：

CUSABIO专为大鼠样本设计的脂多糖/内毒素（LPS）定量检测试剂盒（货号：CSB-E14247r），采用双抗体夹心法原理，可精准检测血清、血浆及组织匀浆样本中0.156 ng/mL至10 ng/mL浓度范围的LPS。LPS作为革兰氏阴性菌细胞壁的关键成分，是引发脓毒症、全身炎症反应综合征等病理过程的重要生物标志物，其定量检测对感染性疾病机制研究、药物疗效评估及免疫调节实验具有重要科研价值。试剂盒适用于实验室环境中动物模型的炎症指标监测，可支持细菌感染研究、内毒素血症模型构建及抗炎药物开发等科研场景。操作流程严格匹配酶标仪检测系统，配套标准品及预包被板实现高灵敏度检测，组织样本需经匀浆预处理后参与检测。本产品仅用于科学研究，检测数据需结合实验设计进行生物学意义解析。
缩写：

LPS
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.156 ng/mL-10 ng/mL
灵敏度：

0.039 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat LPS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-100
1:2	Average %	102
	Range %	96-107
1:4	Average %	98
	Range %	90-106
1:8	Average %	88
	Range %	82-104

回收率：

The recovery of rat LPS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	84-100
EDTA plasma (n=4)	104	100-108

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	1.987	1.999	1.993	1.819
5	1.395	1.357	1.376	1.202
2.5	0.887	0.897	0.892	0.718
1.25	0.595	0.576	0.586	0.412
0.625	0.391	0.384	0.388	0.214
0.312	0.301	0.324	0.313	0.139
0.156	0.247	0.261	0.254	0.080
0	0.173	0.175	0.174

本试剂盒所含材料：
- A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-Rat LPS antibody.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled LPS antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
- One vial Sample Diluent (50 ml/bottle) ---Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) ---Cover the microplate when incubating.
- An instruction manual
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本试剂盒不含材料：
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.