Bovine lipopolysaccharides (LPS) ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO牛脂多糖/内毒素（LPS）酶联免疫试剂盒（货号：CSB-EQ027975BO），采用双抗体夹心法原理，专为科研领域定量检测血清、血浆及组织匀浆样本中的LPS含量而设计。LPS作为革兰氏阴性菌细胞壁的关键成分，可通过激活免疫信号通路引发炎症反应，是研究宿主-病原体相互作用、感染性疾病机制及免疫调节的重要靶标。本试剂盒检测灵敏度高，线性范围为0.312 ng/mL至20 ng/mL，能够精准覆盖低浓度样本的检测需求，适用于动物模型（如牛、小鼠等）中LPS水平的动态监测，例如评估细菌感染后内毒素释放量、炎症反应程度或药物干预效果等研究。实验操作便捷，配套试剂齐全，可为体外实验或动物实验提供可靠的数据支持，助力科研人员在免疫学、微生物学及药理学等领域探索LPS相关分子机制。
缩写：

LPS
种属：

Bos taurus (Bovine)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.312 ng/mL-20 ng/mL
灵敏度：

0.078 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine LPS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	99
	Range %	93-104
1:2	Average %	102
	Range %	98-110
1:4	Average %	90
	Range %	85-94
1:8	Average %	95
	Range %	90-99

回收率：

The recovery of bovine LPS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	80-90
EDTA plasma (n=4)	89	84-93

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.