Rat Leukotriene B4,LT-B4 ELISA Kit

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中文名称：

大鼠白三烯B4(LTB4)酶联免疫试剂盒
货号：

CSB-E08035r
规格：

96T/48T
价格：

￥3900/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO大鼠白三烯B4（LTB4）酶联免疫检测试剂盒（货号：CSB-E08035r），适用于血清、血浆及组织匀浆样本中LTB4含量的精准定量分析。LTB4作为重要的炎症介质，由花生四烯酸代谢产生，在急慢性炎症反应、免疫调节及过敏性疾病机制研究中具有重要价值。该试剂盒采用双抗体夹心法原理，通过预包被特异性抗体实现目标分子的高灵敏度捕获，检测区间覆盖15.6-1000 pg/mL的宽动态范围，可准确反映样本中LTB4的浓度梯度。试剂盒包含标准品、检测抗体及显色系统等完整组分，操作流程标准化，适用于实验室环境中开展炎症相关疾病模型研究、药物干预效果评估或免疫调控机制探索等科研场景。实验样本兼容性强，尤其适合同步分析血液样本与组织局部炎症介质的表达水平，为深入解析LTB4在病理生理过程中的作用提供可靠工具。本产品严格遵循科研用途设计，实验数据需结合其他研究方法进行综合验证。
别名：

N/A
缩写：

LT-B4
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

3.9 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat LT-B4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

Range %

89-99

1:2

Average %

Range %

91-103

1:4

Average %

Range %

92-104

1:8

Average %

Range %

88-97

回收率：

The recovery of rat LT-B4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-96
EDTA plasma (n=4)	97	93-106

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.442	2.541	2.492	2.320
500	1.887	1.986	1.937	1.765
250	1.282	1.263	1.273	1.101
125	0.830	0.821	0.826	0.654
62.5	0.511	0.502	0.507	0.335
31.2	0.370	0.361	0.366	0.194
15.6	0.264	0.259	0.262	0.090
0	0.171	0.172	0.172

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

引用文献

Cannabigerol as an anti-inflammatory agent altering the level of arachidonic acid derivatives in the colon tissue of rats subjected to a high-fat high-sucrose diet K Sztolsztener, E Harasim-Symbor,Biomedicine & Pharmacotherapy,2024
N-acetylcysteine acts as a potent anti-inflammatory agent altering the eicosanoid profile in the development of simple steatosis and its progression to hepatitis K Sztolsztener,/,2023
Palmatine treats urticaria by reducing inflammation and increasing autophagy T Xiao,Frontiers in immunology,2023
α-Lipoic acid–A promising agent for attenuating inflammation and preventing steatohepatitis in rats fed a high-fat diet M Zwierz,Archives of biochemistry and biophysics,2023
Quinoa and Chia Modulate AMPK/PPAR-ɣ Signaling in High-Fat Diet–Induced Obesity Rat Model NH Omran,Revista Brasileira de Farmacognosia,2023
Orally administered solasodine, a steroidal glycoalkaloid, suppresses ovalbumin-induced exaggerated Th2-immune response in rat model of bronchial asthma P Arora,Chemico-biological interactions,2022
Yan-Hou-Qing formula attenuates ammonia-induced acute pharyngitis in rats via inhibition of NF-κB and COX-2 M Xu,BMC Complementary Medicine and Therapies,2020
Enhanced lipoxygenase/LTD4 signaling accounts for the exaggerated hypertensive and nephrotoxic effects of cyclosporine plus indomethacin in rats Helmy MW.et al,Biomedicine & Pharmacotherapy,2018

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Rat Leukotriene B4,LT-B4 ELISA Kit

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