Mouse Leukotriene B4,LT-B4 ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO专用于科研领域的小鼠白三烯B4（LTB4）酶联免疫检测试剂盒（货号：CSB-E08034m），可定量检测血清、血浆、细胞培养上清及组织匀浆样本中LTB4的浓度水平。白三烯B4是花生四烯酸代谢途径中的重要炎症介质，参与中性粒细胞趋化、血管通透性调节等炎症反应过程。试剂盒采用双抗体夹心法原理，预包被特异性抗体可捕获样本中的LTB4，再通过酶标二抗与底物显色系统实现定量检测，其检测灵敏度为15.6 pg/mL，线性范围为15.6-1000 pg/mL，适用于体外实验中对炎症机制、免疫调节或药物干预效果的评估研究。试剂盒包含预包被96孔板、标准品、检测抗体、HRP标记链霉亲和素等核心组分，操作流程包含样本预处理、孵育、洗涤和显色步骤，建议在2-8℃避光保存各组分以确保稳定性。该检测体系可为炎症性疾病模型研究、免疫调控机制探索等科研项目提供可靠的实验数据支持。
别名：

N/A
缩写：

LT-B4
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

3.9 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse LT-B4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-104
1:2	Average %	91
	Range %	86-97
1:4	Average %	95
	Range %	91-99
1:8	Average %	95
	Range %	89-99

回收率：

The recovery of mouse LT-B4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-101
EDTA plasma (n=4)	93	89-97

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.