Rat Keratin, type I cytoskeletal 18 fragment (K18-Asp396）ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

大鼠细胞角蛋白18片段Asp396(K18-Asp396)酶联免疫试剂盒（CSB-E13708r）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CK 18M30含量。试剂盒检测范围为1.56 mIU/mL-100 mIU/mL，适用于基础科研中动态监测细胞凋亡水平、评估药物干预效果或探究疾病模型中上皮细胞损伤机制。适用于肝脏疾病模型研究、肿瘤生物学实验或毒理学评估等非临床科研场景，为细胞死亡相关分子机制研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

N/A
缩写：

CK 18M30
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

1.56 mIU/mL-100 mIU/mL
灵敏度：

0.39 mIU/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat CK 18-M30 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
Range %	91-102
1:2	Average %	94
Range %	90-98
1:4	Average %	84
Range %	80-88
1:8	Average %	103
Range %	99-112

回收率：

The recovery of rat CK 18-M30 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-94
EDTA plasma (n=4)	93	89-97

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.