Human Keratin, type I cytoskeletal 18 fragment (K18-Asp396）ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

人细胞角蛋白18片段Asp396(K18-Asp396)酶联免疫试剂盒（CSB-E13696h）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物.样本中的CK 18M30含量。试剂盒检测范围为15.6 mIU/ml-1000 mIU/ml，适用于体外研究细胞凋亡信号通路、评估药物肝毒性引起的上皮细胞损伤、探索炎症性疾病中组织修复机制等科研实验本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

N/A
缩写：

CK 18M30
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates, cell lysates.
检测范围：

15.6 mIU/ml-1000 mIU/ml
灵敏度：

3.9 mIU/ml
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human CK 18-M30 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
Range %	85-94
1:2	Average %	86
Range %	81-92
1:4	Average %	94
Range %	90-99
1:8	Average %	89
Range %	83-96

回收率：

The recovery of human CK 18-M30 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	99	94-105

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.