Rat Interleukin 1α,IL-1α ELISA kit

Rat Interleukin 1α,IL-1α ELISA kit

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中文名称：

大鼠白介素1α(IL-1α)酶联免疫试剂盒
货号：

CSB-E04622r
规格：

96T/48T
价格：

￥3200/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO夹心法酶联免疫试剂盒，专用于定量检测大鼠血清、血浆及组织匀浆样本中的白介素1α（IL-1α）含量。IL-1α作为重要的促炎细胞因子，广泛参与炎症反应、免疫调节及细胞信号传导等生理病理过程，在感染性疾病、组织修复和免疫相关研究中具有重要价值。试剂盒采用双抗体夹心原理，包被高特异性抗体捕获目标蛋白，通过酶标抗体与底物显色系统实现精准定量，检测灵敏度达78 pg/ml，线性范围覆盖78-5000 pg/ml。适用于科研领域中对炎症机制、免疫调控模型及药物干预效果的评估，尤其适合同时分析体液样本（血清/血浆）与组织局部IL-1α表达水平。样本前处理简单，操作流程标准化，可满足体外实验中多类型生物样本的高通量检测需求，为研究IL-1α在先天免疫应答、细胞应激反应及病理状态下的动态变化提供可靠工具。
别名：

IL1A ELISA Kit; Interleukin-1 alpha ELISA Kit; IL-1 alpha ELISA Kit
缩写：

IL1A
Uniprot No.：

P04822
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates.
检测范围：

78 pg/ml-5000 pg/ml.
灵敏度：

19.5 pg/ml.
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	96
	Range %	92-102
1:2	Average %	98
	Range %	91-104
1:4	Average %	101
	Range %	98-107
1:8	Average %	89
	Range %	84-96

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

Corrected

5000

2.657

2.556

2.607

2.425

2500

2.123

2.022

2.073

1.891

1250

1.468

1.447

1.458

1.276

625

0.927

0.916

0.922

0.740

312

0.568

0.579

0.574

0.392

156

0.415

0.404

0.410

0.228

0.312

0.300

0.306

0.124

0.180

0.183

0.182

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Appraisal of the Pre-Emptive Effect of Lactoferrin Against Chromium-Induced Testicular Toxicity in Male Rats SS Abd El-Rahman,Biological trace element research,2023
Hepatoprotective Potential of Genkwanin Against Aflatoxin B1-Induced Biochemical, Inflammatory and Histopathological Toxicity in Rats MU Ijaz,Pakistan Veterinary Journal,2022
Electroacupuncture Prevents Osteoarthritis of High-Fat Diet-Induced Obese Rats X Lin-Lin,BIOMED RESEARCH INTERNATIONAL,2020
Volatile Oil of Amomum villosum Inhibits Nonalcoholic Fatty Liver Disease via the Gut-Liver Axis Shanhong Lu.et al,BioMed Research International,2018
Magnesium isoglycyrrhizinate inhibits inflammatory response through STAT3 pathway to protect remnant liver function Tang GH. et al,World J Gastroenterol,2015
Ethyl-p-methoxycinnamate isolated from kaempferia galanga inhibits inflammation by suppressing interleukin-1, tumor necrosis factor-a, and angiogenesis by blocking endothelial functions. Umar MI et al,Clinics,2014

功能：

Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
基因功能参考文献：
1. We propose that induction of collagenase-1 by IL-1alpha in both WF and NF depends on a unique combination of cell type-specific signaling pathways. PMID: 17455222
亚细胞定位：

Cytoplasm. Secreted.
蛋白家族：

IL-1 family
数据库链接：

KEGG: ocu:100009250

STRING: 9986.ENSOCUP00000007573

UniGene: Ocu.2080

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.