Rabbit Interleukin 1,IL-1 ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO专为科研设计的兔子白介素1（IL-1）酶联免疫检测试剂盒（货号：CSB-E06896Rb），采用双抗体夹心法定量检测血清、血浆及组织匀浆样本中IL-1的蛋白表达水平。IL-1作为重要的促炎细胞因子，在炎症反应、免疫调控及细胞增殖等生理病理过程中发挥核心作用，其动态变化常被用于研究感染性疾病、自身免疫疾病及组织损伤修复机制。该试剂盒检测灵敏度高，线性范围为15.6-1000 pg/mL，可精准覆盖生物样本中IL-1的常见浓度梯度，配套标准品提供完整定量曲线构建支持。预包被96孔板及优化试剂体系显著缩短操作时间，适用于基础研究中对多组样本进行批量检测，例如探索炎症模型动物血清标志物变化、评估药物干预后组织内细胞因子表达差异等场景。实验全程无需复杂设备，仅需酶标仪即可完成读数分析，为实验室开展免疫调控机制研究提供可靠工具。
别名：

IL1A ELISA Kit; Interleukin-1 alpha ELISA Kit; IL-1 alpha ELISA Kit
缩写：

IL-1
Uniprot No.：

P04822
种属：

Oryctolagus cuniculus (Rabbit)
样本类型：

serum, plasma, tissue homogenates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

3.9 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	84-96
1:2	Average %	92
	Range %	85-100
1:4	Average %	95
	Range %	89-101
1:8	Average %	93
	Range %	87-99

回收率：

The recovery of rabbit IL-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	88-99
EDTA plasma (n=4)	92	84-102

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
基因功能参考文献：
1. We propose that induction of collagenase-1 by IL-1alpha in both WF and NF depends on a unique combination of cell type-specific signaling pathways. PMID: 17455222
亚细胞定位：

Cytoplasm. Secreted.
蛋白家族：

IL-1 family
数据库链接：

KEGG: ocu:100009250

STRING: 9986.ENSOCUP00000007573

UniGene: Ocu.2080