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中文名称：大鼠透明质酸(HA)酶联免疫试剂盒
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货号：CSB-E08120r
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规格：96T/48T
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价格：￥3900/￥2500
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其他：
产品详情
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产品描述：CUSABIO专用于科研领域的大鼠透明质酸（HA）酶联免疫检测试剂盒（货号：CSB-E08120r），采用双抗体夹心法定量检测血清、血浆或组织匀浆样本中的HA含量。透明质酸是细胞外基质的关键成分，参与组织修复、炎症调控及细胞迁移等生物学过程，其浓度变化与肝纤维化、关节炎等疾病模型的研究密切相关。该试剂盒检测灵敏度为4.69 ng/mL，线性范围为4.69-300 ng/mL，适用于探索HA在组织再生、慢性炎症或代谢性疾病中的动态变化，如通过检测肝病模型中的血清HA水平分析纤维化进展，或评估关节炎动物模型中滑膜组织匀浆的HA代谢异常。实验操作采用预包被特异性抗体的96孔板，通过标准品梯度建立定量曲线，配合显色反应实现目标物精准测定，为体外实验提供可靠数据支持。
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别名：N/A
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缩写：HA
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种属：Rattus norvegicus (Rat)
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样本类型：serum, plasma, tissue homogenates
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检测范围：4.69 ng/mL-300 ng/mL
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灵敏度：1.17 ng/mL
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反应时间：1-5h
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样本体积：50-100ul
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检测波长：450 nm
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研究领域：Cell Biology
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测定原理：quantitative
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测定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
线性度：
To assess the linearity of the assay, samples were spiked with high concentrations of rat HA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 93 Range % 90-96 1:2 Average % 91 Range % 87-96 1:4 Average % 97 Range % 93-100 1:8 Average % 89 Range % 85-96 -
回收率：
The recovery of rat HA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 93 90-96 EDTA plasma (n=4) 95 90-101 -
标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
ng/ml OD1 OD2 Average Corrected 300 2.656 2.678 2.667 2.505 150 1.848 1.892 1.870 1.708 75 1.202 1.264 1.233 1.071 37.5 0.763 0.778 0.771 0.609 18.75 0.496 0.473 0.485 0.323 9.38 0.384 0.371 0.378 0.216 4.69 0.275 0.298 0.287 0.125 0 0.161 0.163 0.162 -
数据处理：
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货期：3-5 working days
引用文献
- Chitosan tamarind-based nanoparticles as a promising approach for topical application of curcumin intended for burn healing: in vitro and in vivo study ES Shalaby,Journal of drug targeting,2023
- Effects of novel Fufang Biejia Ruangan Tablets with sheep placenta as substitute for Hominis Placenta on CCl4-induced liver fibrosis B Shen,Chinese Herbal Medicines,2023
- In vivo assessment of the durable, green and in situ bio-functional cotton fabrics based carboxymethyl chitosan nanohybrid for wound healing application TI Shaheen,International journal of biological macromolecules,2023
- Calcium dobesilate prevents PLD-induced hand-foot syndrome by alleviating capillary endothelial tight junction injury via the HA/CD44 pathway C Li,American journal of cancer research,2023
- Cold plasma approach fortifies the topical application of thymoquinone intended for wound healing via up-regulating the levels of TGF-ß, VEGF, and α-SMA in rats AA Sedik,International immunopharmacology,2023
- A standardized pomegranate fruit extract ameliorates thioacetamide-induced liver fibrosis in rats via AGE-RAGE-ROS signaling HM Abouelezz,Heliyon,2023
- Ischemic Preconditioning Alleviates Cerebral Ischemia–Reperfusion Injury by Interfering With Glycocalyx YN Zhang,Translational Stroke Research,2022
- Comprehensive analysis of transcriptomics and metabolomics to illustrate the underlying mechanism of helenalin against hepatic fibrosis Yuhua Xiong,European Journal of Pharmacology,2022
- Mesenchymal stem cell-based Smad7 gene therapy for experimental liver cirrhosis DN Su,Stem Cell Research & Therapy,2020
- Sidr honey abrogates the oxidative stress and downregulates the hyaluronic acid concentration and gene expression of TGF‐β1 and COL1a1 in rat model of thioacetamide‐induced hepatic fibrosis MM Zeweil,Animal Science,2020
- The application of Compont gel in chronic obstructive jaundice rats model Luo WW,ACTA CIRURGICA BRASILEIRA,2019
- The liver sinusoidal endothelial cell damage in rats caused by heatstroke Xingqin Zhang.et al,European Journal of Inflammation,2018
- Hepatoprotective effects of Dicliptera chinensis polysaccharides on dimethylnitrosamine-induced hepatic fibrosis rats and its underlying mechanism Zhang K. et al,J Ethnopharmacol,2015
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