Mouse Hyaluronic acid,HA ELISA Kit

Mouse Hyaluronic acid,HA ELISA Kit

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中文名称：

小鼠透明质酸(HA)酶联免疫试剂盒
货号：

CSB-E08121m
规格：

96T/48T
价格：

￥3800/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO小鼠透明质酸（HA）定量检测试剂盒（货号：CSB-E08121m），采用双抗体夹心ELISA原理，通过预包被特异性捕获抗体与样本中的HA结合，再利用生物素标记的检测抗体形成复合物，结合链霉亲和素-HRP系统进行信号放大，最终通过显色反应实现精准定量分析。其检测范围为62.5-4000 pg/mL，可灵敏检测血清、血浆及组织匀浆等多种样本类型，适用于科研领域对HA表达水平的动态监测。透明质酸作为细胞外基质的重要成分，广泛参与关节润滑、细胞迁移调控及组织修复等生理病理过程，在关节炎模型、皮肤再生研究、肿瘤微环境分析及代谢相关疾病机制探索中具有重要研究价值。本试剂盒提供优化的标准品及酶结合物，操作流程约需4.5小时，实验全程无需特殊设备，96孔板设计支持高通量检测。建议实验前对组织样本进行匀浆离心处理，液体样本避免反复冻融以确保数据稳定性，适用于比较不同病理状态下HA分泌水平差异或评估干预措施对HA代谢的影响。
别名：

N/A
缩写：

HA
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

62.5 pg/mL-4000 pg/mL
灵敏度：

15.6 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cell Biology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse HA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	89
	Range %	82-99
1:200	Average %	93
	Range %	89-101
1:400	Average %	102
	Range %	98-106
1:800	Average %	97
	Range %	92-102

回收率：

The recovery of mouse HA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-94
EDTA plasma (n=4)	97	93-107

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
4000	2.755	2.856	2.806	2.652
2000	2.027	2.128	2.078	1.924
1000	1.435	1.455	1.445	1.291
500	0.850	0.861	0.856	0.702
250	0.526	0.515	0.521	0.367
125	0.358	0.348	0.353	0.199
62.5	0.267	0.261	0.264	0.110
0	0.154	0.153	0.154

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Targeting hyaluronan synthesis enhances the therapeutic effectiveness of biologics in inflammatory bowel disease P Xiao, Z Chen, X Cai, W Xia, X Liu, Z Song,JCI insight,2025
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Dietary supplementation with α-ionone alleviates chronic UVB exposure-induced skin photoaging in mice R Geng,Food & function,2024
Dietary Isoeugenol Supplementation Attenuates Chronic UVB-Induced Skin Photoaging and Modulates Gut Microbiota in Mice Y Chen,Nutrients,2024
Revitalizing Photoaging Skin through Eugenol in UVB-Exposed Hairless Mice: Mechanistic Insights from Integrated Multi-Omics F Feng,Antioxidants,2024
Farnesoid X receptor activation is required for the anti-inflammatory and anti-oxidative stress effects of Alisol B 23-acetate in carbon tetrachloride-induced liver fibrosis in mice L Zhang,International immunopharmacology,2023
Anti-inflammatory and Antifibrotic Potential of Longidaza in the Bleomycin-induced Pulmonary Fibrosis A Pakhomova,/,2023
Combination leflunomide and methotrexate impedes the recovery of liver fibrosis, partly through inhibition of myeloid cell admittance Mengxin Lin, et al,Molecular Medicine Reports,2019
A Polysaccharide from Amusium Pleuronectes Combined with Praziquantel Treatment Ameliorates Hepatic Fibrosis in Schistosoma Japonicum-Infected Mice Tang X.et al,Med Sci Monit,2018
Toxoplasma gondii GRA15II effector-induced M1 cells ameliorate liver fibrosis in mice infected with Schistosomiasis japonica. Xie Y.et al,Cell Mol Immunol.,2016
Role of Hematopoietic Stem Cells in Inflammation of the Pancreas during Diabetes Mellitus. Dygai AM.et al,Bull Exp Biol Med.,2016

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.