Rat High density lipoprotein,HDL ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO大鼠高密度脂蛋白（HDL）酶联免疫检测试剂盒（货号：CSB-E11703r），采用双抗体夹心法定量检测血清、血浆、细胞培养上清液及组织匀浆样本中的HDL含量。HDL作为胆固醇逆向转运的关键载体，在维持脂代谢平衡和心血管健康中具有重要作用，其水平变化与动脉粥样硬化等病理机制密切相关。本试剂盒覆盖78 ng/mL至5000 ng/mL的宽线性检测范围，通过特异性捕获抗体与标记抗体的双重识别，确保检测的高灵敏度和准确性，适用于心血管疾病模型研究、脂代谢机制探索、药物筛选或营养干预评估等科研场景。实验操作流程标准化，用户仅需100 μL样本即可在4.5小时内完成检测，配套试剂包含预包被酶标板、标准品、显色液及终止液等完整组分，支持科研人员高效获取大鼠HDL动态变化的可靠数据。
别名：

N/A
缩写：

HDL
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

78 ng/mL-5000 ng/mL
灵敏度：

19.5 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat HDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	99
	Range %	96-102
1:400	Average %	103
	Range %	100-107
1:800	Average %	89
	Range %	84-96
1:1600	Average %	96
	Range %	94-97

回收率：

The recovery of rat HDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	87-99
EDTA plasma (n=4)	103	99-107

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.