Mouse High density lipoprotein,HDL ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO小鼠高密度脂蛋白（HDL）酶联免疫检测试剂盒（货号CSB-E12840m），采用双抗体夹心法原理实现定量分析，适用于检测血清、血浆及组织匀浆样本中的HDL含量，检测灵敏度达31.25 ng/mL，线性范围覆盖31.25 ng/mL至2000 ng/mL。HDL作为脂蛋白的重要组成成分，在胆固醇逆向转运和心血管健康调控中发挥关键作用，其水平变化常与脂质代谢异常、动脉粥样硬化等病理过程相关。本试剂盒通过特异性捕获抗体与酶标检测抗体的协同作用，可稳定识别小鼠HDL靶标，操作流程标准化，适用于科研领域中对小鼠模型脂代谢机制、心血管疾病病理研究或药物干预效果的评价。实验兼容多种生物样本类型，用户可根据研究需求选择血清、血浆或组织样本进行检测，为探索HDL在生理及疾病状态下的功能提供可靠工具。
缩写：

HDL
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

31.25 ng/mL-2000 ng/mL
灵敏度：

7.8 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse HDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	88
	Range %	84-92
1:2000	Average %	94
	Range %	90-98
1:4000	Average %	99
	Range %	95-104
1:8000	Average %	102
	Range %	96-108

回收率：

The recovery of mouse HDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-96
EDTA plasma (n=4)	100	95-105

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.