Rat Granulocyte-Macrophage Colony Stimulating Factor,GM-CSF ELISA Kit

Rat Granulocyte-Macrophage Colony Stimulating Factor,GM-CSF ELISA Kit

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中文名称：

大鼠粒细胞巨噬细胞集落刺激因子(GM-CSF)酶联免疫试剂盒
货号：

CSB-E04570r
规格：

96T/48T
价格：

￥3900/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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CUSABIO大鼠粒细胞巨噬细胞集落刺激因子（GM-CSF）酶联免疫检测试剂盒（货号：CSB-E04570r），采用双抗体夹心法原理实现定量分析，可精准检测大鼠血清、血浆及组织匀浆样本中GM-CSF的浓度变化，检测范围为15.6 pg/ml至1000 pg/ml。GM-CSF是一种重要的造血生长因子，参与调节粒细胞和巨噬细胞的增殖分化，并在炎症反应、免疫调控及组织修复中发挥关键作用。该试剂盒通过高特异性抗体对靶标进行捕获与检测，适用于科研领域中对大鼠感染性疾病模型、免疫调控机制、造血功能研究以及药物干预效果的评价。实验操作兼容多种生物样本类型，可满足体外实验中GM-CSF的动态监测需求，为探索免疫相关疾病的分子机制或评估实验处理对细胞因子表达的影响提供可靠工具，广泛应用于基础医学、药理学及免疫学等研究场景。
别名：

Csf2 ELISA kit; CsfgmGranulocyte-macrophage colony-stimulating factor ELISA kit; GM-CSF ELISA kit; Colony-stimulating factor ELISA kit; CSF ELISA kit
缩写：

CSF2
Uniprot No.：

P48750
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

15.6 pg/ml - 1000 pg/ml
灵敏度：

3.9 pg/ml
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat GM-CSF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	85
	Range %	81-89
1:2	Average %	97
	Range %	92-101
1:4	Average %	91
	Range %	86-95
1:8	Average %	103
	Range %	99-107

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

Corrected

1000

2.468

2.326

2.397

2.210

500

1.857

1.792

1.825

1.638

250

1.213

1.250

1.232

1.045

125

0.785

0.813

0.799

0.612

62.5

0.492

0.468

0.480

0.293

31.2

0.331

0.347

0.339

0.152

15.6

0.289

0.271

0.280

0.093

0.184

0.189

0.187

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

功能：

Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
基因功能参考文献：
1. Transplant vasculopathy depends on the simultaneous and complementary additive effects of granulocyte-macrophage colony-stimulating factor and cytokines, which have a continuous "cross talk." PMID: 28550897
2. Mesenteric mesothelial cells express GM-CSF receptor on the plasma membrane; during inflammation they synthesize and secrete GM-CSF that can stimulate GM-CSF receptor expression and mesothelial cell to macrophage-like cell transformation; in this transformation, GM-CSF (together with its receptor and other cytokines) has a major autocrine regulatory role; receptor-ligand internalization is essential for GM-CSF signalling PMID: 27364613
3. The findings that ephrinA1-PE38/GM-CSF had a high efficacy in the activation of the dendritic cells would facilitate the development of in vivo dendritic-cell vaccines for the treatment of gliomas in rats PMID: 25677907
4. Monocyte chemotactic protein-1 and GM-CSF may play an important role in the progression of experimental autoimmune myocarditis PMID: 12071442
5. GM-CSF exerts a neuromodulatory effect and may provide a new link of neuroimmune communication. PMID: 14557677
6. We identified the exclusive expression of granulocyte-macrophage colony-stimulating factor (GM-CSF) in Paneth cells using single-cell reverse transcription-polymerase chain reaction and cDNA array. PMID: 14651956
7. GM-CSF may play a significant role in follicular development PMID: 15958400
8. GM-CSF signaling in the central nervous system can regulate energy homeostasis. PMID: 16276414
9. GM-CSF signaling as reflected by PU.1 expression and nuclear binding was restored with recombinant GM-CSF treatment in vitro. PMID: 16877635
10. GM-CSF acts as a neuroprotective protein in the CNS PMID: 17457367
11. Maternal administration of GM-CSF induced fetal lung maturation and this effect may be mediated, at least partly, by an increase in the eNOS expression. PMID: 17478029
12. GM-CSF stimulates neuronal differentiation of adult neural stem cells. PMID: 17953750
13. G-CSF improves and GM-CSF exacerbates early postinfarction LV remodeling in association with modulation of DC infiltration. PMID: 18832728
14. IL-1beta promotes tissue catabolism and destruction, whereas GM-CSF enhances tissue reconstruction PMID: 19022682
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亚细胞定位：

Secreted.
蛋白家族：

GM-CSF family
数据库链接：

KEGG: rno:116630

STRING: 10116.ENSRNOP00000034235

UniGene: Rn.44285

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.