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  4. Rat Granulocyte Colony Stimulating Factor,G-CSF ELISA Kit

Rat Granulocyte Colony Stimulating Factor,G-CSF ELISA Kit

文献引用 (3) 说明书下载
  • 中文名称：
    大鼠粒细胞集落刺激因子(G-CSF)酶联免疫试剂盒
  • 货号：
    CSB-E07340r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO大鼠粒细胞集落刺激因子（G-CSF）定量检测试剂盒，适用于科研领域中对血清、血浆、细胞培养上清液及组织匀浆样本中G-CSF含量的精准分析。G-CSF是一种重要的造血生长因子，在免疫调节中发挥关键作用，能够促进中性粒细胞前体细胞的增殖与分化，并参与炎症反应及感染修复过程。该试剂盒采用双抗体夹心法原理，检测灵敏度可达31.25 pg/mL，线性检测范围为31.25-2000 pg/mL，可覆盖绝大多数实验样本的浓度需求。其高特异性抗体组合能够有效避免交叉反应，确保检测结果的可靠性。该试剂盒在基础研究中可用于探索免疫系统调控机制、炎症相关疾病模型中的G-CSF动态变化，或评估药物/化合物对中性粒细胞生成的影响。例如在感染性疾病模型、骨髓抑制恢复研究或组织损伤修复实验中，可通过检测G-CSF表达水平揭示其生物学功能。试剂盒操作流程标准化，配备预包被96孔板及优化反应体系，适用于实验室常规批量样本检测需求。
  • 别名：
    Csf2 ELISA kit; CsfgmGranulocyte-macrophage colony-stimulating factor ELISA kit; GM-CSF ELISA kit; Colony-stimulating factor ELISA kit; CSF ELISA kit
  • 缩写：
    G-CSF
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    31.25 pg/mL-2000 pg/mL
  • 灵敏度：
    7.81 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat G-CSF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %91
    Range %86-94
    1:2Average %101
    Range %97-104
    1:4Average %95
    Range %90-97
    1:8Average %100
    Range %95-103
  • 回收率：
    The recovery of rat G-CSF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9490-96
    EDTA plasma (n=4)9492-96
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    20002.133 2.118 2.126 2.007
    10001.837 1.776 1.807 1.688
    5001.294 1.282 1.288 1.169
    2500.889 0.878 0.884 0.765
    1250.588 0.607 0.598 0.479
    62.50.361 0.349 0.355 0.236
    31.250.222 0.216 0.219 0.100
    00.118 0.120 0.119 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

产品评价

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靶点详情

  • 功能：
    Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
  • 基因功能参考文献：
    1. Transplant vasculopathy depends on the simultaneous and complementary additive effects of granulocyte-macrophage colony-stimulating factor and cytokines, which have a continuous "cross talk." PMID: 28550897
    2. Mesenteric mesothelial cells express GM-CSF receptor on the plasma membrane; during inflammation they synthesize and secrete GM-CSF that can stimulate GM-CSF receptor expression and mesothelial cell to macrophage-like cell transformation; in this transformation, GM-CSF (together with its receptor and other cytokines) has a major autocrine regulatory role; receptor-ligand internalization is essential for GM-CSF signalling PMID: 27364613
    3. The findings that ephrinA1-PE38/GM-CSF had a high efficacy in the activation of the dendritic cells would facilitate the development of in vivo dendritic-cell vaccines for the treatment of gliomas in rats PMID: 25677907
    4. Monocyte chemotactic protein-1 and GM-CSF may play an important role in the progression of experimental autoimmune myocarditis PMID: 12071442
    5. GM-CSF exerts a neuromodulatory effect and may provide a new link of neuroimmune communication. PMID: 14557677
    6. We identified the exclusive expression of granulocyte-macrophage colony-stimulating factor (GM-CSF) in Paneth cells using single-cell reverse transcription-polymerase chain reaction and cDNA array. PMID: 14651956
    7. GM-CSF may play a significant role in follicular development PMID: 15958400
    8. GM-CSF signaling in the central nervous system can regulate energy homeostasis. PMID: 16276414
    9. GM-CSF signaling as reflected by PU.1 expression and nuclear binding was restored with recombinant GM-CSF treatment in vitro. PMID: 16877635
    10. GM-CSF acts as a neuroprotective protein in the CNS PMID: 17457367
    11. Maternal administration of GM-CSF induced fetal lung maturation and this effect may be mediated, at least partly, by an increase in the eNOS expression. PMID: 17478029
    12. GM-CSF stimulates neuronal differentiation of adult neural stem cells. PMID: 17953750
    13. G-CSF improves and GM-CSF exacerbates early postinfarction LV remodeling in association with modulation of DC infiltration. PMID: 18832728
    14. IL-1beta promotes tissue catabolism and destruction, whereas GM-CSF enhances tissue reconstruction PMID: 19022682

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    GM-CSF family
  • 数据库链接：

    KEGG: rno:116630

    STRING: 10116.ENSRNOP00000034235

    UniGene: Rn.44285

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |