Human Free Testosterone,F-TESTO ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO试剂盒采用竞争法ELISA原理，专用于定量检测人血清或血浆样本中的游离睾酮（F-TESTO），其检测范围为7.5-1500 pg/mL，最低检测限可达7.5 pg/mL，适用于精准分析低浓度样本。游离睾酮作为血液循环中未与性激素结合球蛋白结合的活性形式，直接参与调节男性生殖功能、肌肉合成及内分泌平衡，是研究性激素代谢机制、男性生殖健康及代谢相关疾病的重要生物标志物。本产品通过预包被特异性抗体的微孔板，使样本中的游离睾酮与辣根过氧化物酶标记物竞争结合，经TMB显色后，其吸光度值与目标物浓度呈负相关，结合标准曲线实现定量分析。该试剂盒操作流程约需3.5小时，提供完整的标准品和预混试剂，适用于科研场景下探究内分泌失调、多囊卵巢综合征、代谢综合征等疾病的分子机制，或评估运动生理学、衰老相关激素水平变化等研究，严格限定用于体外实验研究。
缩写：

F-TESTO
种属：

Homo sapiens (Human)
样本类型：

serum, plasma
检测范围：

7.5 pg/mL-1500 pg/mL
灵敏度：

3.75 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Epigenetics and Nuclear Signaling
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human F-TESTO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-102
1:2	Average %	94
	Range %	91-98
1:4	Average %	97
	Range %	93-101
1:8	Average %	94
	Range %	89-98

回收率：

The recovery of human F-TESTO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	92-104
EDTA plasma (n=4)	105	101-109

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.