Rat Fibrinogen,Fbg ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO大鼠血纤蛋白原（Fibrinogen, Fbg）酶联免疫检测试剂盒（货号：CSB-E08201r），基于竞争法原理实现定量分析，适用于检测大鼠血清、血浆及组织匀浆样本中的Fbg含量，检测灵敏度范围为0.312 μg/mL至20 μg/mL。血纤蛋白原是一种由肝脏合成的可溶性血浆糖蛋白，在凝血级联反应中通过转化为纤维蛋白参与血栓形成，同时也是炎症反应和组织修复的重要调控因子，其水平变化常与凝血功能障碍、肝病及炎症性疾病相关。CUSABIO试剂盒采用高特异性抗体，可精准识别大鼠Fbg分子，适用于凝血机制研究、肝损伤模型评估或炎症相关疾病模型的科研场景，例如通过检测血浆样本分析凝血功能异常，或通过组织匀浆检测探究局部炎症反应中Fbg的调控作用。试剂盒操作流程标准化，提供预包被板及配套试剂，兼容多种生物样本类型，满足实验室对Fbg动态变化的定量分析需求，为心血管、肝脏及免疫相关基础研究提供可靠工具。
别名：

N/A
缩写：

Fbg
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.312 μg/mL-20 μg/mL
灵敏度：

0.156 μg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat Fbg in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	99
	Range %	91-103
1:200	Average %	90
	Range %	82-94
1:400	Average %	102
	Range %	91-106
1:800	Average %	101
	Range %	91-105

回收率：

The recovery of rat Fbg spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	93-104
EDTA plasma (n=4)	94	90-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.