Mouse Fibrinogen,Fbg ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO小鼠血纤蛋白原（Fibrinogen, Fbg）酶联免疫检测试剂盒（货号：CSB-E08202m），采用双抗体夹心法定量检测血清、血浆或组织匀浆样本中的Fbg水平，检测灵敏度为0.156 μg/mL，线性范围为0.156-10 μg/mL。血纤蛋白原是凝血系统中的关键糖蛋白，由肝脏合成并在凝血酶作用下转化为纤维蛋白，参与血栓形成、炎症反应和组织修复等生理病理过程。本试剂盒通过特异性抗体捕获样本中的Fbg，结合HRP标记的检测抗体进行显色反应，配合酶标仪测定吸光度值，实现目标蛋白的精确定量。适用于小鼠凝血机制研究、血栓性疾病模型评估、肝脏功能分析或创伤修复相关实验，可为心血管疾病、出血性疾病及炎症反应等科研领域提供可靠的检测工具。试剂盒严格验证抗体特异性及检测稳定性，支持多种生物样本类型，操作流程标准化，满足实验室对Fbg表达水平的高通量检测需求。
缩写：

Fbg
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.156 μg/mL-10 μg/mL
灵敏度：

0.039 μg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse Fbg in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	95
	Range %	87-103
1:400	Average %	92
	Range %	84-105
1:800	Average %	96
	Range %	83-106
1:1600	Average %	93
	Range %	88-104

回收率：

The recovery of mouse Fbg spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	90-108
EDTA plasma (n=4)	96	88-107

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.