Rat Fibrinogen Degradation Product,FDP ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO专为大鼠样本设计的血纤蛋白原降解产物（FDP）定量检测试剂盒，采用双抗体夹心ELISA原理，适用于血清、血浆及组织匀浆样本的科研分析。FDP是纤维蛋白原在纤溶酶作用下的降解产物，其浓度变化可反映机体纤溶系统活性，常用于评估凝血功能异常、血栓性疾病或炎症反应相关研究。试剂盒检测范围为15.6-1000 ng/mL，实验操作中需使用50μL标准品或样本，通过链霉亲和素-HRP系统显色后在450nm波长下测定吸光度值。产品包含预包被96孔板、标准品、检测抗体、显色底物及终止液等完整组分，可在4-8°C保存6个月，避免反复冻融。该试剂盒适用于心血管疾病模型、肝纤维化研究或创伤修复实验中纤溶系统功能的动态监测，为探究凝血与纤溶平衡机制提供高灵敏度的定量工具，实验结果需结合专业分析软件进行标准曲线拟合计算。
别名：

N/A
缩写：

FDP
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

15.6 ng/mL-1000 ng/mL
灵敏度：

3.9 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat FDP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	93-102
1:2	Average %	90
	Range %	85-94
1:4	Average %	95
	Range %	91-99
1:8	Average %	85
	Range %	81-92

回收率：

The recovery of rat FDP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-96
EDTA plasma (n=4)	105	99-109

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.