Pig Fibrinogen Degradation Product,FDP ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO猪血纤蛋白原降解产物（FDP）定量检测试剂盒，采用双抗体夹心酶联免疫吸附法（Sandwich ELISA），可精准分析血清、血浆或组织匀浆样本中的FDP含量。FDP是纤溶系统激活后纤溶酶降解纤维蛋白原或纤维蛋白产生的片段，其水平升高与凝血功能异常、炎症反应或组织损伤修复过程密切相关，是研究猪凝血-纤溶平衡及病理机制的重要指标。试剂盒检测范围为1.25-80 μg/mL，标准曲线线性优异，灵敏度达1.25 μg/mL，可满足不同浓度样本的检测需求。实验操作简便，提供预包被特异性抗体的96孔板及优化试剂体系，支持科研领域对猪凝血相关疾病模型（如血栓形成、弥散性血管内凝血）、药物干预效果评估或器官损伤修复机制的研究。本产品严格验证了与猪样本的特异性结合能力，适用于体外实验，不用于临床诊断。
缩写：

FDP
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates
检测范围：

1.25 μg/mL-80 μg/mL
灵敏度：

0.31 μg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig FDP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-95
1:2	Average %	97
	Range %	92-102
1:4	Average %	90
	Range %	87-98
1:8	Average %	92
	Range %	85-96

回收率：

The recovery of pig FDP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-97
EDTA plasma (n=4)	98	95-101

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.