Rat Dehydroepiandrosterone,DHEA ELISA Kit

Rat Dehydroepiandrosterone,DHEA ELISA Kit

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中文名称：

大鼠脱氢表雄酮(DHEA)酶联免疫试剂盒
货号：

CSB-E08227r
规格：

96T/48T
价格：

￥3900/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO大鼠脱氢表雄酮（DHEA）酶联免疫检测试剂盒（货号：CSB-E08227r），采用竞争法原理实现定量分析，适用于血清、血浆及组织匀浆样本中DHEA含量的精准检测。DHEA是肾上腺分泌的重要类固醇前体激素，参与性激素合成并调控代谢与免疫功能，在生殖生理和衰老研究中具有重要意义。试剂盒检测灵敏度达0.05 ng/mL，线性范围为0.05-10 ng/mL，可覆盖常规实验需求。试剂盒包含预包被板、标准品、HRP标记的检测抗体及显色底物，操作流程优化后可在3小时内完成检测。该产品适用于动物模型中的内分泌研究，如代谢综合征、多囊卵巢综合征的机制探索，或用于评估药物干预对肾上腺功能的影响。试剂盒需-20℃避光保存，开封后标准品需分装冻存避免反复冻融，有效期12个月。实验过程需配合酶标仪（450nm波长）及标准实验室耗材完成，建议初次使用前通过预实验验证样本处理流程。
缩写：

DHEA
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.05 ng/mL-10 ng/mL
灵敏度：

0.025 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Neuroscience
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat DHEA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	102
	Range %	98-107
1:2	Average %	92
	Range %	88-96
1:4	Average %	89
	Range %	85-93
1:8	Average %	100
	Range %	97-104

回收率：

The recovery of rat DHEA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-92
EDTA plasma (n=4)	94	88-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
10	0.162	0.158	0.160
2.5	0.307	0.298	0.303
0.8	0.487	0.504	0.496
0.2	0.801	0.810	0.806
0.05	1.224	1.305	1.265
0	2.000	1.899	1.950

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Constant light exposure alters gut microbiota and short‐/medium‐chain fatty acids and aggravates PCOS‐like traits in HFD‐fed rats F Yue,Obesity,2023

■ 常见问题解答

Please provide the following information regarding Rat Dehydroepiandrosterone, DHEA ELISA Kit (CSB-E08227r): What is the active ingredient of the stop solution and its concentration?
What is the type, concentration, and state (liquid/lyophilized) of the preservatives used?
What is the expression host of the standard?
which components have H202 and the concentration of H202 in them?Just to confirm this I am inquiring for 24 and 96 well of this kit. Please confirm if the information will be the same for both the 24T and 96T in regards to Rat Dehydroepiandrosterone, DHEA ELISA Kit (CSB-E08227r)?
**if the preservative/s are not liquid, what is the concentration of the component prior to reconstitution (while still lyophilized)?

Thanks for your inquiry.
CSB-E08227r
Standard is purified DHEA.
Stop solution is 4N sulfuric acid.
Preservative is proclin 300(1:3000 dilution）.
Sorry we can not disclose which components have H202 and the concentration of H202 in them.
For 24T and 96T, the component information is the same. Only the volume of reagents is different.
Pls let me know if you have any further questions. Thank you.

Can you please clarify if the preservative is liquid or lyophilized?
If it's lyophilized, please let us know concentration too.

Thanks for your further inquiry. It is liquid.

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Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.