Human Dehydroepiandrosterone,DHEA ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO人脱氢表雄酮（DHEA）定量检测专用ELISA试剂盒（货号：CSB-E08226h），采用竞争法检测原理，适用于血清、血浆样本中DHEA的精准分析。DHEA作为肾上腺分泌的重要甾体激素前体，参与睾酮和雌激素的合成代谢，其水平变化与内分泌调节、生殖健康及衰老研究密切相关。试剂盒检测灵敏度达0.1 ng/mL，线性范围覆盖0.1-20 ng/mL，可满足生物样本中DHEA的宽浓度检测需求。实验基于竞争结合原理，样本中的DHEA与固定化标准品竞争结合特异性抗体，通过显色强度反比定量目标物浓度，操作流程包含标准品配制、样本孵育、酶标反应及终止检测等标准化步骤。该试剂盒适用于内分泌代谢研究、生殖系统功能评估、衰老机制探索等科研领域，特别适用于激素水平动态监测、药物干预效果评价等实验场景。配套提供预包被酶标板、检测抗体、标准品及全套实验试剂，支持科研人员高效开展人血清或血浆样本检测，实验全程无需复杂样本前处理，3.5小时内即可完成检测流程。
缩写：

DHEA
种属：

Homo sapiens (Human)
样本类型：

serum, plasma
检测范围：

0.1 ng/mL-20 ng/mL
灵敏度：

0.05 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Neuroscience
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human DHEA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	91-110
1:2	Average %	93
	Range %	87-100
1:4	Average %	95
	Range %	89-104
1:8	Average %	90
	Range %	85-100

回收率：

The recovery of human DHEA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-102
EDTA plasma (n=4)	96	92-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.