Rat Creatine Kinase MM isoenzyme,CK-MM ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

大鼠肌酸激酶同工酶MM(CK-MM)酶联免疫试剂盒（CSB-E14405r）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CK-MM含量。CK-MM即肌酸激酶同工酶，主要存在于骨骼肌中。它可作为诊断急性心肌梗死等疾病的重要指标，反映心肌和骨骼肌损伤情况。研究机制方面，通过检测血液中CK-MM活性变化，能辅助判断病情严重程度与预后，为临床治疗提供关键依据。试剂盒检测范围为7.8 μU/mL-500 μU/mL，适用于运动医学、肌肉疾病模型构建、药物毒理评估等科研场景中CK - MM的动态监测。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

CK-MM
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

7.8 μU/mL-500 μU/mL
灵敏度：

1.95 μU/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat CK-MM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	105
	Range %	101-109
1:200	Average %	87
	Range %	84-90
1:400	Average %	102
	Range %	98-106
1:800	Average %	105
	Range %	101-109

回收率：

The recovery of rat CK-MM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	87	85-90

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.