Mouse Creatine Kinase MM isoenzyme,CK-MM ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

小鼠肌酸激酶同工酶MM(CK-MM)酶联免疫试剂盒（CSB-E14407m）为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的CK-MM含量。CK-MM即肌酸激酶同工酶，主要存在于骨骼肌和心肌中，在临床上是诊断急性心肌梗死等疾病的重要指标。研究机制方面，通过检测血液中CK-MM的含量变化，可反映心肌或骨骼肌细胞损伤情况，在心肌梗死时其水平会快速升高。试剂盒检测范围为0.156 mU/mL-10 mU/mL，适用于基础科研中骨骼肌损伤模型评估、运动生理代谢研究、肌肉相关疾病机制探索等场景，例如通过监测血清CK-MM浓度变化研究肌肉再生能力或病理损伤程度。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

CK-MM
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma
检测范围：

0.156 mU/mL-10 mU/mL
灵敏度：

0.039 mU/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse CK-MM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	83-98
1:2	Average %	98
	Range %	92-103
1:4	Average %	104
	Range %	98-108
1:8	Average %	93
	Range %	87-99

回收率：

The recovery of mouse CK-MM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	85-99
EDTA plasma (n=4)	100	95-105

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.