Rat Collagen Type Ⅳ,Col Ⅳ ELISA KIT

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO大鼠Ⅳ型胶原（Col Ⅳ）定量检测专用酶联免疫试剂盒，采用双抗体夹心原理，适用于血清、血浆及离心处理后的组织匀浆样本检测。Ⅳ型胶原是细胞外基质核心成分，主要分布于基底膜结构，参与组织修复与纤维化进程，其异常表达与器官纤维化及血管病变密切相关。试剂盒线性检测范围为1.56-100 ng/mL，灵敏度达1.56 ng/mL，操作全程可在4.5小时内完成，提供预包被板、标准品、检测抗体及显色系统等完整组分，标准曲线R²值＞0.99，确保数据可靠性。该试剂盒可应用于肝/肾纤维化动物模型构建、血管基底膜损伤研究、肿瘤微环境分析等科研领域，特别适用于评估疾病模型中Col Ⅳ的动态变化及组织损伤修复机制，为心血管疾病、器官纤维化等病理过程的分子机制研究提供精准检测工具。所有组分经严格质控，包含标准操作规程及技术指导文件，支持96孔板规格批量检测。
别名：

Collagen Type 4,Col 4,_x000D_ Collagen Type IV,Col IV
缩写：

Col Ⅳ
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

1.56 ng/mL-100 ng/mL
灵敏度：

0.39 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat COL Ⅳ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-105
1:2	Average %	96
	Range %	92-101
1:4	Average %	92
	Range %	87-96
1:8	Average %	87
	Range %	82-94

回收率：

The recovery of rat COL Ⅳ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	104	99-108

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.