Mouse Collagen Type Ⅳ,Col Ⅳ ELISA KIT

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO小鼠Ⅳ型胶原（Col Ⅳ）酶联免疫检测试剂盒（货号：CSB-E08884m），采用双抗体夹心法定量检测血清、血浆及组织匀浆样本中的Col Ⅳ含量，检测灵敏度达0.78 ng/mL，线性范围为0.78-50 ng/mL。Ⅳ型胶原作为基底膜的核心结构蛋白，广泛参与组织形态维持、细胞黏附及信号调控，其表达水平变化与纤维化病变、肿瘤微环境重塑及损伤修复等病理生理过程密切相关。本试剂盒通过高特异性抗体对实现精准定量，适用于科研领域中小鼠模型的相关研究，例如通过检测血清/血浆中Col Ⅳ浓度评估全身性基底膜代谢状态，或通过分析肝、肾等组织匀浆的Col Ⅳ表达水平，探究器官特异性纤维化进展及药物干预效果。试剂盒操作便捷，可满足体外实验中对Col Ⅳ动态变化的定量分析需求，为基底膜相关机制研究提供可靠工具。
别名：

Collagen Type 4,Col 4,_x000D_ Collagen Type IV,Col IV
缩写：

Col Ⅳ
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.78 ng/mL-50 ng/mL
灵敏度：

0.195 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse COL Ⅳ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	100
	Range %	97-103
1:2	Average %	89
	Range %	84-93
1:4	Average %	88
	Range %	84-92
1:8	Average %	101
	Range %	97-106

回收率：

The recovery of mouse COL Ⅳ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	81-90
EDTA plasma (n=4)	96	93-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.